UniProt Accession

 PHAX_RAT; Q63068; Q4QQW9; Q5RJZ7 

Genbank Protein ID

 X67877; BC086410; BC097932 

Genbank Nucleotide ID

 CAA48076.1; AAH86410.1; AAH97932.1 

Protein Name

 Phosphorylated adapter RNA export protein 

Protein Synonyms/Alias

 26 kDa resiniferatoxin-binding protein; RBP-26; RNA U small nuclear RNA export adapter protein; RTX-42; Resiniferatoxin-binding protein 2; RBP-2 

Gene Name

 Phax 

Gene Synonyms/Alias

 Rnuxa 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
148	TYNYLLAKKLAKKES	acetylation	[1]
323	RRTQLLGKKMKEAIK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation- independent manner. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)- capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA (By similarity). 

Sequence Annotation

 REGION 2 320 Necessary for interaction with CBP80 (By
 REGION 219 319 Sufficient for poly U RNA-binding (By
 REGION 270 278 Necessary for poly U RNA-binding and
 MOTIF 71 74 Nuclear localization signal (By
 MOTIF 120 129 Nuclear export signal (By similarity).
 MOTIF 189 192 Nuclear localization signal (By
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 14 14 Phosphoserine (By similarity).
 MOD_RES 287 287 Phosphothreonine (By similarity).
 MOD_RES 347 347 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Protein transport; Reference proteome; RNA-binding; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 385 AA 

Protein Sequence

Gene Ontology

 GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IDA:RGD.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0015643; F:toxic substance binding; IPI:RGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006408; P:snRNA export from nucleus; IEA:Compara. 

Interpro

 IPR019385; PHAX_RNA-binding_domain. 

Pfam

 PF10258; RNA_GG_bind 

SMART

  

PROSITE

  

PRINTS