CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000583
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 Ech1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
76VQLNRPEKRNAMNRAacetylation[1, 2, 3]
94ELVECFQKISKDSDCacetylation[1, 2, 3]
97ECFQKISKDSDCRAVacetylation[1]
111VVVSGAGKMFTSGIDacetylation[4]
111VVVSGAGKMFTSGIDsuccinylation[4]
147YLRDLISKYQKTFTVacetylation[1, 2]
147YLRDLISKYQKTFTVubiquitination[5]
150DLISKYQKTFTVIEKacetylation[1, 2, 3]
150DLISKYQKTFTVIEKubiquitination[5]
230ELTFSARKMMADEALubiquitination[5]
275PVAVQGSKINLIYSRubiquitination[5]
316SVQAAMEKRDTKSITacetylation[1]
320AMEKRDTKSITFSKLacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA (By similarity). 
Sequence Annotation
 MOTIF 325 327 Microbody targeting signal (Potential).
 BINDING 173 173 Substrate; via amide nitrogen (By
 MOD_RES 326 326 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Fatty acid metabolism; Isomerase; Lipid metabolism; Mitochondrion; Peroxisome; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 327 AA 
Protein Sequence
MATAMTVSSK LRGLLMQQLR GTSQLYFNIS LRSLSSSAQE ASKRAPEEVS DHNYESIQVT 60
SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD CRAVVVSGAG KMFTSGIDLM 120
DMASELMQPS GDDAARIAWY LRDLISKYQK TFTVIEKCPK PVIAAIHGGC IGGGVDLVSA 180
CDIRYCTQDA FFQIKEVDMG LAADVGTLQR LPKVIGNQSL VNELTFSARK MMADEALDSG 240
LVSRVFQDKD AMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM 300
LQTQDIIKSV QAAMEKRDTK SITFSKL 327 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
 GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR014748; Crontonase_C.
 IPR001753; Crotonase_core_superfam.
 IPR018376; Enoyl-CoA_hyd/isom_CS. 
Pfam
 PF00378; ECH 
SMART
  
PROSITE
 PS00166; ENOYL_COA_HYDRATASE 
PRINTS