CPLM-002604

Genbank Nucleotide ID

Protein Synonyms/Alias

67 kDa calelectrin; Annexin VI; Annexin-6; Calphobindin-II; CPB-II; Chromobindin-20; Lipocortin VI; Protein III; p68; p70

Homo sapiens (Human)

Position	Peptide	Type	References
9	AKPAQGAKYRGSIHD	acetylation	[1]
9	AKPAQGAKYRGSIHD	ubiquitination	[2]
40	MKGFGSDKEAILDII	ubiquitination	[2]
63	QEVCQSYKSLYGKDL	acetylation	[3]
63	QEVCQSYKSLYGKDL	ubiquitination	[2, 4]
68	SYKSLYGKDLIADLK	acetylation	[3]
68	SYKSLYGKDLIADLK	ubiquitination	[2, 5, 6]
75	KDLIADLKYELTGKF	acetylation	[3]
81	LKYELTGKFERLIVG	acetylation	[3]
81	LKYELTGKFERLIVG	ubiquitination	[2, 4, 5, 6]
99	PPAYCDAKEIKDAIS	ubiquitination	[2]
102	YCDAKEIKDAISGIG	ubiquitination	[2, 7]
113	SGIGTDEKCLIEILA	ubiquitination	[2]
135	HQLVAAYKDAYERDL	ubiquitination	[2, 4, 5, 6, 8]
220	LVFDEYLKTTGKPIE	ubiquitination	[2]
224	EYLKTTGKPIEASIR	ubiquitination	[2]
240	ELSGDFEKLMLAVVK	ubiquitination	[2]
247	KLMLAVVKCIRSTPE	ubiquitination	[2]
299	IFRTKYEKSLYSMIK	acetylation	[3]
306	KSLYSMIKNDTSGEY	acetylation	[3]
306	KSLYSMIKNDTSGEY	ubiquitination	[2]
370	FNPDADAKALRKAMK	acetylation	[3]
377	KALRKAMKGLGTDED	ubiquitination	[2, 4, 5, 6, 7, 8, 9]
406	QQIRQTFKSHFGRDL	ubiquitination	[2]
418	RDLMTDLKSEISGDL	acetylation	[3, 10]
418	RDLMTDLKSEISGDL	ubiquitination	[2, 7]
442	PPAHYDAKQLKKAME	acetylation	[3]
446	YDAKQLKKAMEGAGT	ubiquitination	[2]
456	EGAGTDEKALIEILA	ubiquitination	[2]
478	RAINEAYKEDYHKSL	ubiquitination	[2]
483	AYKEDYHKSLEDALS	acetylation	[3]
483	AYKEDYHKSLEDALS	ubiquitination	[2]
600	IVQSVKNKPLFFADK	ubiquitination	[2, 4]
607	KPLFFADKLYKSMKG	acetylation	[3]
607	KPLFFADKLYKSMKG	ubiquitination	[2]
620	KGAGTDEKTLTRIMV	acetylation	[3]
620	KGAGTDEKTLTRIMV	ubiquitination	[2]
647	EFIEKYDKSLHQAIE	ubiquitination	[2]

[1] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[9] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[10] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]

Functional Description

May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

REPEAT 29 89 Annexin 1.
REPEAT 101 161 Annexin 2.
REPEAT 185 245 Annexin 3.
REPEAT 260 320 Annexin 4.
REPEAT 372 432 Annexin 5.
REPEAT 444 504 Annexin 6.
REPEAT 533 593 Annexin 7.
REPEAT 608 668 Annexin 8.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 30 30 Phosphotyrosine.
MOD_RES 63 63 N6-acetyllysine.
MOD_RES 68 68 N6-acetyllysine.
MOD_RES 75 75 N6-acetyllysine.
MOD_RES 81 81 N6-acetyllysine.
MOD_RES 201 201 Phosphotyrosine (By similarity).
MOD_RES 306 306 N6-acetyllysine.
MOD_RES 370 370 N6-acetyllysine.
MOD_RES 418 418 N6-acetyllysine.
MOD_RES 483 483 N6-acetyllysine.
MOD_RES 620 620 N6-acetyllysine.

3D-structure; Acetylation; Alternative splicing; Annexin; Calcium; Calcium/phospholipid-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB.
GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
GO:0005509; F:calcium ion binding; IEA:InterPro.
GO:0005544; F:calcium-dependent phospholipid binding; IDA:UniProtKB.
GO:0006816; P:calcium ion transport; IEA:Compara.
GO:0006937; P:regulation of muscle contraction; IEA:Compara.

IPR001464; Annexin.
IPR018502; Annexin_repeat.
IPR018252; Annexin_repeat_CS.
IPR002393; AnnexinVI.

PR00196; ANNEXIN.
PR00202; ANNEXINVI.