CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009086
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 25 
Protein Synonyms/Alias
 Deubiquitinating enzyme 25; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25; mUSP25 
Gene Name
 Usp25 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
57AVAFLTAKNAKTPPQubiquitination[1]
201RRLVLNYKPPSNAQDubiquitination[1, 2]
250SRAVEILKDAFKSNDubiquitination[1]
254EILKDAFKSNDSQQQubiquitination[1]
312AMGVLEGKKFENTEMubiquitination[1]
313MGVLEGKKFENTEMFubiquitination[1]
392RPEKIHNKLEFPQVLubiquitination[1]
444LSYGSGPKRFPLVDVubiquitination[1, 2]
521PLRSVIHKPFTQSRIubiquitination[1]
585IELMYSDKSMIQVPYubiquitination[1, 2]
623HRESRWMKYNDIAVTubiquitination[1]
631YNDIAVTKSSWEELVubiquitination[1]
699EDNQRFEKELEEWDTubiquitination[1]
716AQRSLQEKLLAAPKLubiquitination[1]
722EKLLAAPKLREAEASacetylation[3]
722EKLLAAPKLREAEASubiquitination[1]
790LEYSRLVKLAQEDTPubiquitination[1]
1011KLTDFLPKLLDCSTEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains (By similarity). 
Sequence Annotation
 DOMAIN 14 57 UBA-like.
 REPEAT 97 116 UIM 1.
 REPEAT 123 140 UIM 2.
 REGION 77 102 SUMO interaction domain (SIM).
 MOTIF 89 95 Required for SUMO paralog-specific
 ACT_SITE 178 178 Nucleophile (By similarity).
 ACT_SITE 608 608 Proton acceptor (By similarity).
 CROSSLNK 99 99 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 99 99 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Hydrolase; Isopeptide bond; Nucleus; Phosphoprotein; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1055 AA 
Protein Sequence
MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDAQILQQAL KDSNGNLELA VAFLTAKNAK 60
TPPQEETGYY QTALPGNDRY ISVGSQADAN VIDLTGDDKD DLQRAIALSL AESNRAFRET 120
GITDEEQAIS RVLEASIAEN KACLKRTPIE VWRDSRNPYD RKRQEKAPVG LKNVGNTCWF 180
SAVIQSLFNL LEFRRLVLNY KPPSNAQDLP RNQKEHRNLP FMRELRYLFA LLVGTKRKYV 240
DPSRAVEILK DAFKSNDSQQ QDVSEFTHKL LDWLEDAFQM KAEEETDEEK PKNPMVELFY 300
GRFLAMGVLE GKKFENTEMF GQYPLQVNGF KDLHECLEAA MIEGEIESLH SDNSGKSGQE 360
HWFTELPPVL TFELSRFEFN QALGRPEKIH NKLEFPQVLY LDRYMHRNRE ITRIKREEIK 420
RLKDYLTVLQ QRLERYLSYG SGPKRFPLVD VLQYALEFAS SKPVCTSPVD DIDASSSASG 480
PLPSQSLPST TEQQGPCASD LPGSSSPASG AALPLRSVIH KPFTQSRIPP DLPMHPAPRH 540
ITEEELCVLE SCLHRWRTEI ENDTRDLQES ISRIHRTIEL MYSDKSMIQV PYRLHAVLVH 600
EGQANAGHYW AYIFDHRESR WMKYNDIAVT KSSWEELVRD SFGGYRNASA YCLMYIDDKA 660
QFLIQEEFNK ETGQALVGME TLPPDLRDFV EEDNQRFEKE LEEWDTQLAQ RSLQEKLLAA 720
PKLREAEASA TTAQAGGADY LEQPSRSDLS KHWKEETLRV IAKASHDLED KGPETVLQSA 780
IKLEYSRLVK LAQEDTPPET DYRLHHVLVY FIQNQAPKKI IEKTLLEQFG DRNLSFDERC 840
HNIMKVAQAK LEMIKPEEVN LEEYEEWHAD YKKFRETTMY LITGLENFQR ESYIDSLLFL 900
LCAYQNNKEL LSKGPYRGHD GELISHYRRE CLLKLNEQAA ELFESGEDGD VNNGLIIMNE 960
FIVPFLPLLL VDDMEEKDIL AVEDMRNRWC SYLGQEMEAN LQEKLTDFLP KLLDCSTEIK 1020
GFHEPPKLPS YSAHELCERF ARIMLSLSRT PADGR 1055 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000502; C:proteasome complex; ISS:MGI.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:MGI.
 GO:0071108; P:protein K48-linked deubiquitination; IEA:Compara.
 GO:0070536; P:protein K63-linked deubiquitination; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:MGI. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR009060; UBA-like.
 IPR003903; Ubiquitin-int_motif. 
Pfam
 PF00443; UCH
 PF02809; UIM 
SMART
 SM00726; UIM 
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3
 PS50330; UIM 
PRINTS