CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044032
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Replication factor C subunit 2 
Protein Synonyms/Alias
  
Gene Name
 RFC2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19RTMEIYSKTTRFALAubiquitination[1, 2]
32LACNASDKIIEPIQSacetylation[3]
32LACNASDKIIEPIQSubiquitination[2, 3]
48CAVLRYTKLTDAQILacetylation[3, 4]
48CAVLRYTKLTDAQILubiquitination[1, 2, 3, 5, 6]
64RLMNVIEKERVPYTDubiquitination[1, 2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 198 AA 
Protein Sequence
XMTDGAQQAL RRTMEIYSKT TRFALACNAS DKIIEPIQSR CAVLRYTKLT DAQILTRLMN 60
VIEKERVPYT DDGLEAIIFT AQGDMRQALN NLQSTFSGFG FINSENVFKV CDEPHPLLVK 120
EMIQHCVNAN IDEAYKILAH LWHLGYSPED IIGNIFRVCK TFQMAEYLKL EFIKELPNGF 180
PKWLQHVHSH QHSGGSST 198 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0006260; P:DNA replication; IEA:InterPro. 
Interpro
 IPR008921; DNA_pol3_clamp-load_cplx_C.
 IPR027417; P-loop_NTPase.
 IPR013748; Rep_factorC_C_dom. 
Pfam
 PF08542; Rep_fac_C 
SMART
  
PROSITE
  
PRINTS