UniProt Accession

 CETN2_HUMAN; P41208; B2R4T4; Q53XW1 

Genbank Protein ID

 X72964; U82671; AK311940; BT007256; AY919675; CH471172; BC005334; BC013873 

Genbank Nucleotide ID

 CAA51467.1; BAG34881.1; AAP35920.1; AAW82436.1; EAW72900.1; AAH05334.1; AAH13873.1 

Protein Name

 Centrin-2 

Protein Synonyms/Alias

 Caltractin isoform 1 

Gene Name

 CETN2 

Gene Synonyms/Alias

 CALT; CEN2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
6	**MASNFKKANMASS	ubiquitination	[1]
22	QRKRMSPKPELTEEQ	ubiquitination	[2]
54	TIDVKELKVAMRALG	ubiquitination	[1]
78	KMISEIDKEGTGKMN	ubiquitination	[1]
103	KMSEKDTKEEILKAF	ubiquitination	[1]
108	DTKEEILKAFKLFDD	ubiquitination	[3, 4]
111	EEILKAFKLFDDDET	ubiquitination	[2, 3, 4]
120	FDDDETGKISFKNLK	ubiquitination	[1, 5]
124	ETGKISFKNLKRVAK	ubiquitination	[1, 2, 3, 4, 5]
127	KISFKNLKRVAKELG	ubiquitination	[1]
131	KNLKRVAKELGENLT	ubiquitination	[6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Plays a fundamental role in microtubule organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CEP110. 

Sequence Annotation

 DOMAIN 28 63 EF-hand 1.
 DOMAIN 64 99 EF-hand 2.
 DOMAIN 101 136 EF-hand 3.
 DOMAIN 137 172 EF-hand 4.
 REGION 1 25 Required for self-assembly.
 MOD_RES 20 20 Phosphoserine.
 MOD_RES 26 26 Phosphothreonine.  

Keyword

 3D-structure; Calcium; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; DNA repair; Metal-binding; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 172 AA 

Protein Sequence

Gene Ontology

 GO:0005814; C:centriole; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005932; C:microtubule basal body; IEA:Compara.
 GO:0032391; C:photoreceptor connecting cilium; IEA:Compara.
 GO:0071942; C:XPC complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007099; P:centriole replication; IMP:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007067; P:mitosis; NAS:UniProtKB.
 GO:0006289; P:nucleotide-excision repair; IDA:UniProtKB.
 GO:0032465; P:regulation of cytokinesis; IMP:UniProtKB.
 GO:0007283; P:spermatogenesis; IEA:Compara. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000629; RNA-helicase_DEAD-box_CS. 

Pfam

 PF13499; EF_hand_5 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS