CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001358
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 
Protein Synonyms/Alias
 PAPS synthase 2; PAPSS 2; Sulfurylase kinase 2; SK 2; SK2; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase 
Gene Name
 Papss2 
Gene Synonyms/Alias
 Atpsk2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
174RARAGEIKGFTGIDSubiquitination[1]
185GIDSDYEKPETPECVubiquitination[1]
234ELFVPENKVDQIRAEubiquitination[1]
276LKGFMREKEYLQTLHubiquitination[1]
400RLTPLELKQKCKDMNubiquitination[1]
466VPLEWRMKQHAAVLEubiquitination[1]
540YEPTHGGKVLSMAPGubiquitination[1]
608FMAPKAWKVLTDYYRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. May have a important role in skeletogenesis during postnatal growth. 
Sequence Annotation
 NP_BIND 50 57 ATP (Potential).
 REGION 1 ?211 Adenylyl-sulfate kinase.
 REGION ?212 621 Sulfate adenylyltransferase.
 MOTIF 517 521 PP-motif.
 ACT_SITE 124 124 Phosphoserine intermediate (By  
Keyword
 ATP-binding; Complete proteome; Disease mutation; Kinase; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 621 AA 
Protein Sequence
MSANFKMNHK RDQQKSTNVV YQAHHVSRNK RGQVVGTRGG FRGCTVWLTG LSGAGKTTIS 60
FALEEYLVSH AIPCYSLDGD NVRHGLNKNL GFSAGDREEN IRRIAEVARL FADAGLVCIT 120
SFISPFAKDR ENARKIHESA GLPFFEIFVD APLNICESRD VKGLYKRARA GEIKGFTGID 180
SDYEKPETPE CVLKTNLSSV SDCVQQVVEL LQEQNIVPHT TIKGIHELFV PENKVDQIRA 240
EAETLPSLPI TKLDLQWVQI LSEGWATPLK GFMREKEYLQ TLHFDTLLDG VVPRDGVINM 300
SIPIVLPVSA DDKARLEGCS KFALMYEGRR VALLQDPEFY EHRKEERCSR VWGTATAKHP 360
HIKMVMESGD WLVGGDLQVL ERIRWDDGLD QYRLTPLELK QKCKDMNADA VFAFQLRNPV 420
HNGHALLMQD TRRRLLERGY KHPVLLLHPL GGWTKDDDVP LEWRMKQHAA VLEERVLDPK 480
STIVAIFPSP MLYAGPTEVQ WHCRCRMIAG ANFYIVGRDP AGMPHPETKK DLYEPTHGGK 540
VLSMAPGLTS VEIIPFRVAA YNKIKKAMDF YDPARHEEFD FISGTRMRKL AREGEDPPDG 600
FMAPKAWKVL TDYYRSLEKT N 621 
Gene Ontology
 GO:0004020; F:adenylylsulfate kinase activity; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004781; F:sulfate adenylyltransferase (ATP) activity; ISS:UniProtKB.
 GO:0007596; P:blood coagulation; IMP:MGI.
 GO:0060348; P:bone development; IMP:MGI.
 GO:0000103; P:sulfate assimilation; IDA:MGI. 
Interpro
 IPR002891; APS_kinase.
 IPR025980; ATP-Sase_PUA-like_dom.
 IPR027417; P-loop_NTPase.
 IPR015947; PUA-like_domain.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR024951; Sulfurylase_cat_dom.
 IPR002650; Sulphate_adenylyltransferase. 
Pfam
 PF01583; APS_kinase
 PF01747; ATP-sulfurylase
 PF14306; PUA_2 
SMART
  
PROSITE
  
PRINTS