UniProt Accession

 NAPEP_HUMAN; Q6IQ20; Q5CZ87; Q769K1 

Genbank Protein ID

 AY357337; AB112352; CR936639; CH471070; BC071604 

Genbank Nucleotide ID

 AAR13673.1; BAD02399.1; CAI56779.1; EAW83314.1; AAH71604.1 

Protein Name

 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D 

Protein Synonyms/Alias

 N-acyl phosphatidylethanolamine phospholipase D; NAPE-PLD; NAPE-hydrolyzing phospholipase D 

Gene Name

 NAPEPLD 

Gene Synonyms/Alias

 C7orf18 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
73	VNPWPTWKNPSIPNV	ubiquitination	[1, 2]
97	HSSVPSSKEELDKEL	ubiquitination	[2]
102	SSKEELDKELPVLKP	ubiquitination	[1]
296	PAFEEIGKRFGPFDL	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N- arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors (By similarity). 

Sequence Annotation

 METAL 185 185 Zinc 1 (Potential).
 METAL 187 187 Zinc 1 (Potential).
 METAL 189 189 Zinc 2 (Potential).
 METAL 190 190 Zinc 2 (Potential).
 METAL 253 253 Zinc 1 (Potential).
 METAL 343 343 Zinc 2 (Potential).  

Keyword

 Complete proteome; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Metal-binding; Phospholipid degradation; Phospholipid metabolism; Polymorphism; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 393 AA 

Protein Sequence

Gene Ontology

 GO:0042622; C:photoreceptor outer segment membrane; TAS:Reactome.
 GO:0070290; F:NAPE-specific phospholipase D activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0009395; P:phospholipid catabolic process; IEA:UniProtKB-KW. 

Interpro

 IPR001279; Beta-lactamas-like.
 IPR024884; NAPE-PLD. 

Pfam

  

SMART

  

PROSITE

  

PRINTS