UniProt Accession

 K6PF1_ECOLI; P0A796; P06998; Q2M8L2 

Genbank Protein ID

 X02519; L19201; U00096; AP009048 

Genbank Nucleotide ID

 CAA26356.1; AAB03048.1; AAC76898.1; BAE77394.1 

Protein Name

 6-phosphofructokinase isozyme 1 

Protein Synonyms/Alias

 6-phosphofructokinase isozyme I; Phosphofructokinase 1; Phosphohexokinase 1 

Gene Name

 pfkA 

Gene Synonyms/Alias

 b3916; JW3887 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
91	AVAIENLKKRGIDAL	acetylation	[1]
237	ELAHFIEKETGRETR	acetylation	[1, 2]
305	IDAIENMKRPFKGDW	acetylation	[1]
309	ENMKRPFKGDWLDCA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

  

Sequence Annotation

 NP_BIND 22 26 ATP.
 NP_BIND 155 159 ATP.
 NP_BIND 172 188 ATP.
 ACT_SITE 128 128 Proton acceptor.
 METAL 186 186 Magnesium; via carbonyl oxygen.
 METAL 188 188 Magnesium.
 BINDING 163 163 Substrate.
 BINDING 244 244 Substrate.
 BINDING 250 250 Substrate.
 BINDING 253 253 Substrate.  

Keyword

 3D-structure; Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 320 AA 

Protein Sequence

Gene Ontology

 GO:0005945; C:6-phosphofructokinase complex; IDA:EcoliWiki.
 GO:0003872; F:6-phosphofructokinase activity; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IMP:EcoliWiki.
 GO:0019003; F:GDP binding; IDA:EcoCyc.
 GO:0042802; F:identical protein binding; IDA:EcoliWiki.
 GO:0000287; F:magnesium ion binding; IDA:EcoliWiki.
 GO:0044275; P:cellular carbohydrate catabolic process; IMP:EcoliWiki.
 GO:0006002; P:fructose 6-phosphate metabolic process; IEA:InterPro.
 GO:0006096; P:glycolysis; IDA:EcoliWiki. 

Interpro

 IPR012003; ATP_PFK_prok.
 IPR012828; PFKA_ATP.
 IPR022953; Phosphofructokinase.
 IPR015912; Phosphofructokinase_CS.
 IPR000023; Phosphofructokinase_dom. 

Pfam

 PF00365; PFK 

SMART

  

PROSITE

 PS00433; PHOSPHOFRUCTOKINASE 

PRINTS

 PR00476; PHFRCTKINASE.