CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022329
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sphingomyelin phosphodiesterase 4 
Protein Synonyms/Alias
 Neutral sphingomyelinase 3; nSMase-3; nSMase3; Neutral sphingomyelinase III 
Gene Name
 SMPD4 
Gene Synonyms/Alias
 KIAA1418 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
23LKADSINKPFAQQCQubiquitination[1]
99PMMKLVYKLQAEDYKubiquitination[1]
106KLQAEDYKFDFPVSYubiquitination[1, 2]
238LHHTSLLKRHISHQTubiquitination[1]
460PWRYAPDKQAPGSDSubiquitination[1]
507RTDLVSPKHALMVFRubiquitination[1, 2]
575TDASFKVKSHVYSLEubiquitination[1]
587SLEGQDCKYTPMFGPubiquitination[1]
611AQLITQAKHTAKSISubiquitination[1]
660MGQDSVRKTDEYLEKubiquitination[1]
667KTDEYLEKALEYLRQubiquitination[1, 2, 3]
699TTQDENGKKQLPDCIubiquitination[1]
700TQDENGKKQLPDCIVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. 
Sequence Annotation
 MOD_RES 130 130 Phosphoserine.
 MOD_RES 669 669 Phosphothreonine.  
Keyword
 Alternative splicing; Complete proteome; Endoplasmic reticulum; Golgi apparatus; Hydrolase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 827 AA 
Protein Sequence
MAFPHLQQPS FLLASLKADS INKPFAQQCQ DLVKVIEDFP AKELHTIFPW LVESIFGSLD 60
GVLVGWNLRC LQGRVNPVEY SIVMEFLDPG GPMMKLVYKL QAEDYKFDFP VSYLPGPVKA 120
SIQECILPDS PLYHNKVQFT PTGGLGLNLA LNPFEYYIFF FALSLITQKP LPVSLHVRTS 180
DCAYFILVDR YLSWFLPTEG SVPPPLSSSP GGTSPSPPPR TPAIPFASYG LHHTSLLKRH 240
ISHQTSVNAD PASHEIWRSE TLLQVFVEMW LHHYSLEMYQ KMQSPHAKLE VLHYRLSVSS 300
ALYSPAQPSL QALHAYQESF TPTEEHVLVV RLLLKHLHAF ANSLKPEQAS PSAHSHATSP 360
LEEFKRMGLN LPEVPSALPR RSQPSLMGAL GSSVSRASLL GKQQVTLPIP CRAAVPRFVQ 420
QKLYLFLQHC FGHWPLDASF RAVLEMWLSY LQPWRYAPDK QAPGSDSQPR CVSEKWAPFV 480
QENLLMYTKL FVGFLNRALR TDLVSPKHAL MVFRVAKVFA QPNLAEMIQK GEQLFLEPEL 540
VIPHRQHRLF TAPTFTGSFL SPWPPAVTDA SFKVKSHVYS LEGQDCKYTP MFGPEARTLV 600
LRLAQLITQA KHTAKSISDQ CAESPAGHSF LSWLGFSSMD TNGSYTANDL DEMGQDSVRK 660
TDEYLEKALE YLRQIFRLSE AQLRQFTLAL GTTQDENGKK QLPDCIVGED GLILTPLGRY 720
QIINGLRRFE IEYQGDPELQ PIRSYEIASL VRTLFRLSSA INHRFAGQMA ALCSRDDFLG 780
SFCRYHLTEP GLASRHLLSP VGRRQVAGHT RGPRLSLRFL GSYRTLVSLL LAFFVASLFC 840
VGPLPCTLLL TLGYVLYASA MTLLTERGKL HQP 873 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IDA:HGNC.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005802; C:trans-Golgi network; IDA:HGNC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004767; F:sphingomyelin phosphodiesterase activity; TAS:Reactome.
 GO:0050290; F:sphingomyelin phosphodiesterase D activity; IDA:HGNC.
 GO:0071356; P:cellular response to tumor necrosis factor; IDA:BHF-UCL.
 GO:0046513; P:ceramide biosynthetic process; IDA:BHF-UCL.
 GO:0046475; P:glycerophospholipid catabolic process; IDA:BHF-UCL.
 GO:0006687; P:glycosphingolipid metabolic process; TAS:Reactome.
 GO:0006685; P:sphingomyelin catabolic process; IDA:HGNC. 
Interpro
 IPR024129; Sphingomy_SMPD4. 
Pfam
  
SMART
  
PROSITE
  
PRINTS