CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005347
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteinase-activated receptor 1 
Protein Synonyms/Alias
 PAR-1; Coagulation factor II receptor; Thrombin receptor 
Gene Name
 F2R 
Gene Synonyms/Alias
 CF2R; PAR1; TR 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
421NLNNSIYKKLLT***ubiquitination[1, 2, 3, 4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. 
Sequence Annotation
 CARBOHYD 35 35 N-linked (GlcNAc...) (Potential).
 CARBOHYD 62 62 N-linked (GlcNAc...) (Potential).
 CARBOHYD 75 75 N-linked (GlcNAc...) (Potential).
 CARBOHYD 250 250 N-linked (GlcNAc...) (Potential).
 CARBOHYD 259 259 N-linked (GlcNAc...) (Potential).
 DISULFID 175 254 By similarity.  
Keyword
 3D-structure; Blood coagulation; Cell membrane; Complete proteome; Disulfide bond; G-protein coupled receptor; Glycoprotein; Hemostasis; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 425 AA 
Protein Sequence
MGPRRLLLVA ACFSLCGPLL SARTRARRPE SKATNATLDP RSFLLRNPND KYEPFWEDEE 60
KNESGLTEYR LVSINKSSPL QKQLPAFISE DASGYLTSSW LTLFVPSVYT GVFVVSLPLN 120
IMAIVVFILK MKVKKPAVVY MLHLATADVL FVSVLPFKIS YYFSGSDWQF GSELCRFVTA 180
AFYCNMYASI LLMTVISIDR FLAVVYPMQS LSWRTLGRAS FTCLAIWALA IAGVVPLLLK 240
EQTIQVPGLN ITTCHDVLNE TLLEGYYAYY FSAFSAVFFF VPLIISTVCY VSIIRCLSSS 300
AVANRSKKSR ALFLSAAVFC IFIICFGPTN VLLIAHYSFL SHTSTTEAAY FAYLLCVCVS 360
SISCCIDPLI YYYASSECQR YVYSILCCKE SSDPSSYNSS GQLMASKMDT CSSNLNNSIY 420
KKLLT 425 
Gene Ontology
 GO:0005901; C:caveola; IDA:BHF-UCL.
 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; TAS:ProtInc.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0031594; C:neuromuscular junction; ISS:UniProtKB.
 GO:0031094; C:platelet dense tubular network; IDA:BHF-UCL.
 GO:0045211; C:postsynaptic membrane; ISS:UniProtKB.
 GO:0001965; F:G-protein alpha-subunit binding; ISS:UniProtKB.
 GO:0031681; F:G-protein beta-subunit binding; ISS:UniProtKB.
 GO:0015057; F:thrombin receptor activity; IDA:BHF-UCL.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:BHF-UCL.
 GO:0000186; P:activation of MAPKK activity; ISS:UniProtKB.
 GO:0009653; P:anatomical structure morphogenesis; TAS:ProtInc.
 GO:0002248; P:connective tissue replacement involved in inflammatory response wound healing; IDA:BHF-UCL.
 GO:0051482; P:elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway; ISS:UniProtKB.
 GO:0007529; P:establishment of synaptic specificity at neuromuscular junction; ISS:UniProtKB.
 GO:0048873; P:homeostasis of number of cells within a tissue; ISS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:BHF-UCL.
 GO:0003105; P:negative regulation of glomerular filtration; ISS:UniProtKB.
 GO:0043524; P:negative regulation of neuron apoptotic process; ISS:UniProtKB.
 GO:1900134; P:negative regulation of renin secretion into blood stream; ISS:UniProtKB.
 GO:0030168; P:platelet activation; IDA:BHF-UCL.
 GO:0060155; P:platelet dense granule organization; IC:BHF-UCL.
 GO:0030194; P:positive regulation of blood coagulation; IDA:BHF-UCL.
 GO:0030335; P:positive regulation of cell migration; IMP:BHF-UCL.
 GO:0008284; P:positive regulation of cell proliferation; ISS:UniProtKB.
 GO:0032967; P:positive regulation of collagen biosynthetic process; IDA:BHF-UCL.
 GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IEP:UniProtKB.
 GO:2000778; P:positive regulation of interleukin-6 secretion; IDA:UniProtKB.
 GO:2000484; P:positive regulation of interleukin-8 secretion; IDA:UniProtKB.
 GO:0046427; P:positive regulation of JAK-STAT cascade; IDA:BHF-UCL.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; ISS:UniProtKB.
 GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IDA:BHF-UCL.
 GO:0035025; P:positive regulation of Rho protein signal transduction; ISS:UniProtKB.
 GO:0045987; P:positive regulation of smooth muscle contraction; ISS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0045907; P:positive regulation of vasoconstriction; ISS:UniProtKB.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; ISS:UniProtKB.
 GO:0032651; P:regulation of interleukin-1 beta production; ISS:UniProtKB.
 GO:0051930; P:regulation of sensory perception of pain; ISS:UniProtKB.
 GO:0051209; P:release of sequestered calcium ion into cytosol; ISS:UniProtKB.
 GO:0032496; P:response to lipopolysaccharide; ISS:UniProtKB.
 GO:0007262; P:STAT protein import into nucleus; IDA:BHF-UCL.
 GO:0007260; P:tyrosine phosphorylation of STAT protein; IDA:BHF-UCL. 
Interpro
 IPR000276; GPCR_Rhodpsn.
 IPR017452; GPCR_Rhodpsn_7TM.
 IPR003912; Protea_act_rcpt.
 IPR000935; Thrmbn_rcpt. 
Pfam
 PF00001; 7tm_1 
SMART
  
PROSITE
 PS00237; G_PROTEIN_RECEP_F1_1
 PS50262; G_PROTEIN_RECEP_F1_2 
PRINTS
 PR00237; GPCRRHODOPSN.
 PR01428; PROTEASEAR.
 PR00908; THROMBINR.