CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022690
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Programmed cell death 6-interacting protein 
Protein Synonyms/Alias
 ALG-2-interacting protein 1 
Gene Name
 Pdcd6ip 
Gene Synonyms/Alias
 Aip1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
19TSEVDLAKPLVKFIQacetylation[1]
19TSEVDLAKPLVKFIQubiquitination[2]
23DLAKPLVKFIQQTYPubiquitination[2]
48RAAEELSKLRRSALGacetylation[1]
48RAAEELSKLRRSALGubiquitination[2]
60ALGRPLDKHEGALETacetylation[1]
60ALGRPLDKHEGALETubiquitination[2]
215MKDAIIAKLANQAADacetylation[1]
274ILAKQQKKFGEEIARacetylation[1]
303YDEYVNVKDFSDKINacetylation[1]
344IGKATLVKPTPVNVPacetylation[1]
491TPSNDLYKPLRAEGAacetylation[1]
506KFRAVLDKAVQADGQubiquitination[2, 3]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. 
Sequence Annotation
 DOMAIN 3 397 BRO1.
 REGION 176 508 Interaction with CHMP4A, CHMP4B and
 REGION 508 873 Self-association (By similarity).
 REGION 722 725 Interaction with TSG101 (By similarity).
 REGION 802 811 Interaction with CEP55 (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 215 215 N6-acetyllysine (By similarity).
 MOD_RES 735 735 Phosphoserine (By similarity).
 MOD_RES 742 742 Phosphothreonine (By similarity).
 MOD_RES 745 745 Phosphothreonine (By similarity).  
Keyword
 Acetylation; Apoptosis; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 873 AA 
Protein Sequence
MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR RSALGRPLDK 60
HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD KGSLFGGSVK LALASLGYEK 120
SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA KQYQFASGAF LHIKDTVLSA LSREPTVDIS 180
PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDALPKY 240
FYFQEVFPTL AAKQCIMQAN AEYHQSILAK QQKKFGEEIA RLQHAAELIK NVASRYDEYV 300
NVKDFSDKIN RALAAAKKDN DFIYHDRVPD LKDLDPIGKA TLVKPTPVNV PISQKFTDLF 360
EKMVPVSVQQ SLAVFSQRKA DLVNRSIAQM REATTLANGV LASLNLPAAI EDVSGDTVPQ 420
SILTKSTAVV EQGGIQTVDQ LIKELPELLQ RNREILEESL RLLDEEEATD NDLRAKFKDR 480
WQRTPSNDLY KPLRAEGAKF RAVLDKAVQA DGQVKERYQS HRDTIALLCK PEPELNAAIP 540
SANPAKTMQG SEVVNVLKSL LSNLDEIKKE REGLENDLKS VNFDMTSKFL TALAQDGVIN 600
EEALSVTELD RIYGGLTTKV QESLKKQEGL LKNIQVSHQE FSKMKQSNSE ASLREEVLKN 660
LATAYDNFVE LVANLKEGTK FYNELTEILV RFQNKCSDIV FARKTERDEL LKDLQQSIAR 720
EPSAPSIPPP AYQSSPAGGH ATAPTPAPRT MPPAKPQPPA RPPPPVLPAN RVPPAAAATA 780
PAGVGTASAA PPQTPGSAPP PQAQGPPYPT YPGYPGYCQM PMPMGYNPYT YGQYNMPYPP 840
VYHQSPGQAP YPGPQQPTYP FPQPPQQSYY PQQ 873 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:RGD.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0017124; F:SH3 domain binding; IDA:RGD.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR025304; ALIX_V_dom.
 IPR004328; BRO1_dom. 
Pfam
 PF13949; ALIX_LYPXL_bnd
 PF03097; BRO1 
SMART
 SM01041; BRO1 
PROSITE
 PS51180; BRO1 
PRINTS