CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009697
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform 
Protein Synonyms/Alias
 PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B isoform alpha 
Gene Name
 PPP2R2A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
62FQQEQENKIQSHSRGubiquitination[1, 2, 3]
88EPEFDYLKSLEIEEKacetylation[4]
88EPEFDYLKSLEIEEKubiquitination[2, 3]
98EIEEKINKIRWLPQKacetylation[4]
105KIRWLPQKNAAQFLLubiquitination[1, 2, 3, 5, 6]
117FLLSTNDKTIKLWKIubiquitination[2]
123DKTIKLWKISERDKRubiquitination[6]
129WKISERDKRPEGYNLubiquitination[2, 3]
137RPEGYNLKEEDGRYRubiquitination[2, 3]
267ALCDRHSKLFEEPEDubiquitination[2, 3]
387EASRENNKPRTVLKPubiquitination[3]
404VCASGKRKKDEISVDubiquitination[2]
417VDSLDFNKKILHTAWubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. 
Sequence Annotation
 REPEAT 26 65 WD 1.
 REPEAT 91 132 WD 2.
 REPEAT 175 213 WD 3.
 REPEAT 224 264 WD 4.
 REPEAT 283 321 WD 5.
 REPEAT 338 379 WD 6.
 REPEAT 414 446 WD 7.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 447 AA 
Protein Sequence
MAGAGGGNDI QWCFSQVKGA VDDDVAEADI ISTVEFNHSG ELLATGDKGG RVVIFQQEQE 60
NKIQSHSRGE YNVYSTFQSH EPEFDYLKSL EIEEKINKIR WLPQKNAAQF LLSTNDKTIK 120
LWKISERDKR PEGYNLKEED GRYRDPTTVT TLRVPVFRPM DLMVEASPRR IFANAHTYHI 180
NSISINSDYE TYLSADDLRI NLWHLEITDR SFNIVDIKPA NMEELTEVIT AAEFHPNSCN 240
TFVYSSSKGT IRLCDMRASA LCDRHSKLFE EPEDPSNRSF FSEIISSISD VKFSHSGRYM 300
MTRDYLSVKI WDLNMENRPV ETYQVHEYLR SKLCSLYEND CIFDKFECCW NGSDSVVMTG 360
SYNNFFRMFD RNTKRDITLE ASRENNKPRT VLKPRKVCAS GKRKKDEISV DSLDFNKKIL 420
HTAWHPKENI IAVATTNNLY IFQDKVN 447 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000159; C:protein phosphatase type 2A complex; IDA:UniProtKB.
 GO:0008601; F:protein phosphatase type 2A regulator activity; TAS:UniProtKB.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0007084; P:mitotic nuclear envelope reassembly; TAS:Reactome.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
 GO:0006470; P:protein dephosphorylation; IDA:UniProtKB.
 GO:0043278; P:response to morphine; IEA:Compara.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR000009; PP2A_PR55.
 IPR018067; PP2A_PR55_CS.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
  
SMART
 SM00320; WD40 
PROSITE
 PS01024; PR55_1
 PS01025; PR55_2
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00600; PP2APR55.