CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007411
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Type I restriction enzyme StySJI M protein 
Protein Synonyms/Alias
 M.StySJI 
Gene Name
 hsdM 
Gene Synonyms/Alias
 hsdT; STM4525 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
469SLDISWLKDKDSIDAacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Methylation of specific adenine residues; required for both restriction and modification activities (By similarity). The StySJI enzyme recognizes 5'-GAGN(6)GTRC-3'. 
Sequence Annotation
 REGION 148 153 S-adenosyl-L-methionine binding (By
 REGION 178 180 S-adenosyl-L-methionine binding (By
 BINDING 216 216 S-adenosyl-L-methionine (By similarity).  
Keyword
 Complete proteome; Methyltransferase; Reference proteome; Restriction system; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 529 AA 
Protein Sequence
MNNNDLVAKL WKLCDNLRDG GVSYQNYVNE LASLLFLKMC KETGQEADYL PEGYRWDDLK 60
SRIGQDQMQF YRNLLVQLGS DEKKLVQAVF HNVSTTIEQP KQLTELVSYM DALDWYNGNH 120
GKSRDDFGDM YEGLLQKNAN ETKSGAGQYF TPRPLIKTII HLLKPQPREV VQDPAAGTAG 180
FLIEADRYVK SQTNDLDDLD GDTQDFQIHR AFIGLELVPG TRRLALMNCL LHDIEGNLDH 240
GGAIRLGNTL GSDGENLPKA HIVATNPPFG SAAGTNITRT FVHPTSNKQL CFMQHIIETL 300
HPGGRAAVVV PDNVLFEGGK GTDIRRDLMD KCHLHTILRL PTGIFYAQGV KTNVLFFTKG 360
TVTNPHQDKN CTDDVWVYDL RTNMPSFGKR TPFTEQHLQP FETVYGEDPH GLSPREEGEW 420
SFNAEESEVA DSEENKNTDQ HQATSRWRKF SREWIRSAKS DSLDISWLKD KDSIDADSLP 480
EPDVLAAEAM GELVQALGEL DALMRELGAG DEADAQRQLL NEAFGEVKA 529 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
 GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:EC.
 GO:0032775; P:DNA methylation on adenine; IEA:GOC.
 GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW. 
Interpro
 IPR022749; D12N6_MeTrfase_N.
 IPR003356; DNA_methylase_A-5.
 IPR002052; DNA_methylase_N6_adenine_CS.
 IPR002296; N12N6_MeTrfase. 
Pfam
 PF12161; HsdM_N
 PF02384; N6_Mtase 
SMART
  
PROSITE
 PS00092; N6_MTASE 
PRINTS
 PR00507; N12N6MTFRASE.