CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005688
UniProt Accession
Genbank Protein ID
 D90345 
Genbank Nucleotide ID
Protein Name
 T-complex protein 1 subunit alpha 
Protein Synonyms/Alias
 TCP-1-alpha; CCT-alpha 
Gene Name
 Tcp1 
Gene Synonyms/Alias
 Cct1; Ccta 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
199VNSVNILKAHGRSQIacetylation[1]
400HDALCVVKRVLESKSacetylation[1]
466DSTDLVAKLRAFHNEacetylation[1]
484NPERKNLKWIGLDLVacetylation[1]
494GLDLVHGKPRDNKQAacetylation[1]
538IKLHPESKDDKHGGYacetylation[1]
538IKLHPESKDDKHGGYubiquitination[2]
541HPESKDDKHGGYENAacetylation[1]
541HPESKDDKHGGYENAubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity). 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 181 181 Phosphotyrosine (By similarity).
 MOD_RES 182 182 Phosphothreonine (By similarity).
 MOD_RES 199 199 N6-acetyllysine (By similarity).
 MOD_RES 400 400 N6-acetyllysine (By similarity).
 MOD_RES 551 551 Phosphoserine (By similarity).  
Keyword
 Acetylation; ATP-binding; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 556 AA 
Protein Sequence
MEGPLSVFGD RSTGEAIRSQ NVMAAASIAN IVKSSLGPVG LDKMLVDDIG DVTITNDGAT 60
ILKLLEVEHP AAKVLCELAD LQDKEVGDGT TSVVIIAAEL LKNADELVKQ KIHPTSVISG 120
YRLACKEAVR YINENLIINT DELGRDCLIN AAKTSMSSKI IGINGDFFAN MVVDAVLAVK 180
YTDIRGQPRY PVNSVNILKA HGRSQIESML INGYALNCVV GSQGMLKRIV NAKIACLDFS 240
LQKTKMKLGV QVVITDPEKL DQIRQRESDI TKERIQKILA TGANVILTTG GIDDMCLKYF 300
VEAGAMAVRR VLKRDLKRIA KASGASILST LANLEGEETF EATMLGQAEE VVQERICDDE 360
LILIKNTKAR TSASIILRGA NDFMCDEMER SLHDALCVVK RVLESKSVVP GGGAVEAALS 420
IYLENYATSM GSREQLAIAE FARSLLVIPN TLAVNAAQDS TDLVAKLRAF HNEAQVNPER 480
KNLKWIGLDL VHGKPRDNKQ AGVFEPTIVK VKSLKFATEA AITILRIDDL IKLHPESKDD 540
KHGGYENAVH SGALDD 556 
Gene Ontology
 GO:0030054; C:cell junction; IEA:Compara.
 GO:0005832; C:chaperonin-containing T-complex; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0000792; C:heterochromatin; IDA:RGD.
 GO:0005874; C:microtubule; IEA:Compara.
 GO:0005720; C:nuclear heterochromatin; IEA:Compara.
 GO:0000242; C:pericentriolar material; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006457; P:protein folding; TAS:RGD.
 GO:0007286; P:spermatid development; TAS:RGD. 
Interpro
 IPR012715; Chap_CCT_alpha.
 IPR017998; Chaperone_TCP-1.
 IPR002194; Chaperonin_TCP-1_CS.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial.
 IPR027410; TCP-1-like_intermed. 
Pfam
 PF00118; Cpn60_TCP1 
SMART
  
PROSITE
 PS00750; TCP1_1
 PS00751; TCP1_2
 PS00995; TCP1_3 
PRINTS
 PR00304; TCOMPLEXTCP1.