CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023242
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Syntaxin-8 
Protein Synonyms/Alias
  
Gene Name
 STX8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
59KEKIALLKDLLLRAVubiquitination[1, 2, 3]
98RLLLASFKNEGAEPDubiquitination[1, 3, 4, 5, 6, 7]
117SLMSEEAKRGAPNPWubiquitination[6]
146EIRQQQQKIIQEQDAubiquitination[1, 2, 3, 4, 6, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER. 
Sequence Annotation
 DOMAIN 145 207 t-SNARE coiled-coil homology.  
Keyword
 Coiled coil; Complete proteome; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 236 AA 
Protein Sequence
MAPDPWFSTY DSTCQIAQEI AEKIQQRNQY ERKGEKAPKL TVTIRALLQN LKEKIALLKD 60
LLLRAVSTHQ ITQLEGDRRQ NLLDDLVTRE RLLLASFKNE GAEPDLIRSS LMSEEAKRGA 120
PNPWLFEEPE ETRGLGFDEI RQQQQKIIQE QDAGLDALSS IISRQKQMGQ EIGNELDEQN 180
EIIDDLANLV ENTDEKLRNE TRRVNMVDRK SASCGMIMVI LLLLVAIVVV AVWPTN 236 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0031902; C:late endosome membrane; IEA:Compara.
 GO:0005765; C:lysosomal membrane; IEA:Compara.
 GO:0031201; C:SNARE complex; IEA:Compara.
 GO:0008333; P:endosome to lysosome transport; IEA:Compara.
 GO:0006810; P:transport; TAS:ProtInc.
 GO:0006906; P:vesicle fusion; IEA:Compara. 
Interpro
 IPR015640; Syntax_8.
 IPR000727; T_SNARE_dom. 
Pfam
 PF05739; SNARE 
SMART
 SM00397; t_SNARE 
PROSITE
 PS00914; SYNTAXIN
 PS50192; T_SNARE 
PRINTS