CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019244
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Signal-induced proliferation-associated protein 1 
Protein Synonyms/Alias
 Sipa-1; GTPase-activating protein Spa-1; p130 SPA-1 
Gene Name
 SIPA1 
Gene Synonyms/Alias
 SPA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
294PRGLSPRKLLEHVAPubiquitination[1]
316RLGSASPKVPRTLLTubiquitination[1]
334QVLSFQRKVGILYCRubiquitination[1]
510FRAFLLAKALNGEQAubiquitination[1]
1032TRLLLASKQLGSPTAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state. 
Sequence Annotation
 DOMAIN 321 539 Rap-GAP.
 DOMAIN 687 763 PDZ.
 MOD_RES 64 64 Phosphothreonine.
 MOD_RES 67 67 Phosphoserine.
 MOD_RES 817 817 Phosphoserine.
 MOD_RES 839 839 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; GTPase activation; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1042 AA 
Protein Sequence
MPMWAGGVGS PRRGMAPAST DDLFARKLRQ PARPPLTPHT FEPRPVRGPL LRSGSDAGEA 60
RPPTPASPRA RAHSHEEASR PAATSTRLFT DPLALLGLPA EEPEPAFPPV LEPRWFAHYD 120
VQSLLFDWAP RSQGMGSHSE ASSGTLASAE DQAASSDLLH GAPGFVCELG GEGELGLGGP 180
ASPPVPPALP NAAVSILEEP QNRTSAYSLE HADLGAGYYR KYFYGKEHQN FFGMDESLGP 240
VAVSLRREEK EGSGGGTLHS YRVIVRTTQL RTLRGTISED ALPPGPPRGL SPRKLLEHVA 300
PQLSPSCLRL GSASPKVPRT LLTLDEQVLS FQRKVGILYC RAGQGSEEEM YNNQEAGPAF 360
MQFLTLLGDV VRLKGFESYR AQLDTKTDST GTHSLYTTYQ DHEIMFHVST MLPYTPNNQQ 420
QLLRKRHIGN DIVTIVFQEP GSKPFCPTTI RSHFQHVFLV VRAHTPCTPH TTYRVAVSRT 480
QDTPAFGPAL PAGGGPFAAN ADFRAFLLAK ALNGEQAAGH ARQFHAMATR TRQQYLQDLA 540
TNEVTTTSLD SASRFGLPSL GGRRRAAPRG PGAELQAAGS LVWGVRAAPG ARVAAGAQAS 600
GPEGIEVPCL LGISAEALVL VAPRDGRVVF NCACRDVLAW TFSEQQLDLY HGRGEAITLR 660
FDGSPGQAVG EVVARLQLVS RGCETRELAL PRDGQGRLGF EVDAEGFVTH VERFTFAETA 720
GLRPGARLLR VCGQTLPSLR PEAAAQLLRS APKVCVTVLP PDESGRPRRS FSELYTLSLQ 780
EPSRRGAPDP VQDEVQGVTL LPTTKQLLHL CLQDGGSPPG PGDLAEERTE FLHSQNSLSP 840
RSSLSDEAPV LPNTTPDLLL ATTAKPSVPS ADSETPLTQD RPGSPSGSED KGNPAPELRA 900
SFLPRTLSLR NSISRIMSEA GSGTLEDEWQ AISEIASTCN TILESLSREG QPIPESGDPK 960
GTPKSDAEPE PGNLSEKVSH LESMLRKLQE DLQKEKADRA ALEEEVRSLR HNNRRLQAES 1020
ESAATRLLLA SKQLGSPTAD LA 1042 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IC:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0046582; F:Rap GTPase activator activity; NAS:UniProtKB.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0007010; P:cytoskeleton organization; NAS:UniProtKB.
 GO:0035556; P:intracellular signal transduction; NAS:UniProtKB.
 GO:0007162; P:negative regulation of cell adhesion; NAS:UniProtKB.
 GO:0045786; P:negative regulation of cell cycle; NAS:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; NAS:UniProtKB. 
Interpro
 IPR001478; PDZ.
 IPR000331; Rap_GAP_dom. 
Pfam
 PF00595; PDZ
 PF02145; Rap_GAP 
SMART
 SM00228; PDZ 
PROSITE
 PS50106; PDZ
 PS50085; RAPGAP 
PRINTS