CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005915
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 High affinity cationic amino acid transporter 1 
Protein Synonyms/Alias
 CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; ERR; Solute carrier family 7 member 1; System Y+ basic amino acid transporter 
Gene Name
 SLC7A1 
Gene Synonyms/Alias
 ATRC1; ERR; REC1L 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MGCKVLLNIGQubiquitination[1, 2, 3]
215GFVKGSVKNWQLTEEubiquitination[2, 4, 5]
373AEDGLLFKFLANVNDubiquitination[4]
484LKTILSPKNMEPSKIubiquitination[4, 6, 7]
629DGNLDQCK*******ubiquitination[3, 8, 9]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
 High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues. May also function as an ecotropic retroviral leukemia receptor. 
Sequence Annotation
 CARBOHYD 216 216 N-linked (GlcNAc...); atypical.
 CARBOHYD 226 226 N-linked (GlcNAc...).
 CARBOHYD 235 235 N-linked (GlcNAc...) (Potential).  
Keyword
 Amino-acid transport; Complete proteome; Direct protein sequencing; Glycoprotein; Membrane; Receptor; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 629 AA 
Protein Sequence
MGCKVLLNIG QQMLRRKVVD CSREETRLSR CLNTFDLVAL GVGSTLGAGV YVLAGAVARE 60
NAGPAIVISF LIAALASVLA GLCYGEFGAR VPKTGSAYLY SYVTVGELWA FITGWNLILS 120
YIIGTSSVAR AWSATFDELI GRPIGEFSRT HMTLNAPGVL AENPDIFAVI IILILTGLLT 180
LGVKESAMVN KIFTCINVLV LGFIMVSGFV KGSVKNWQLT EEDFGNTSGR LCLNNDTKEG 240
KPGVGGFMPF GFSGVLSGAA TCFYAFVGFD CIATTGEEVK NPQKAIPVGI VASLLICFIA 300
YFGVSAALTL MMPYFCLDNN SPLPDAFKHV GWEGAKYAVA VGSLCALSAS LLGSMFPMPR 360
VIYAMAEDGL LFKFLANVND RTKTPIIATL ASGAVAAVMA FLFDLKDLVD LMSIGTLLAY 420
SLVAACVLVL RYQPEQPNLV YQMASTSDEL DPADQNELAS TNDSQLGFLP EAEMFSLKTI 480
LSPKNMEPSK ISGLIVNIST SLIAVLIITF CIVTVLGREA LTKGALWAVF LLAGSALLCA 540
VVTGVIWRQP ESKTKLSFKV PFLPVLPILS IFVNVYLMMQ LDQGTWVRFA VWMLIGFIIY 600
FGYGLWHSEE ASLDADQART PDGNLDQCK 629 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0015181; F:arginine transmembrane transporter activity; IEA:Compara.
 GO:0006865; P:amino acid transport; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome. 
Interpro
 IPR002293; AA/rel_permease1.
 IPR015606; Cat-AATrans.
 IPR004755; Cat_AA_permease. 
Pfam
  
SMART
  
PROSITE
  
PRINTS