CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018364
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dynein heavy chain 7, axonemal 
Protein Synonyms/Alias
 Axonemal beta dynein heavy chain 7; Ciliary dynein heavy chain 7; Dynein heavy chain-like protein 2; hDHC2 
Gene Name
 DNAH7 
Gene Synonyms/Alias
 KIAA0944 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
95ERFGKKGKLPHQVDDacetylation[1]
113GPSTSKSKGKSPHKEacetylation[1]
1142KARGQVEKWLVELERacetylation[2]
1157VMINSIHKVTGDATFubiquitination[3]
3718NANADITKDQSETQLacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP (By similarity). 
Sequence Annotation
 NP_BIND 132 139 ATP (Potential).
 NP_BIND 1328 1335 ATP (Potential).
 NP_BIND 1609 1616 ATP (Potential).
 NP_BIND 1976 1983 ATP (Potential).
 NP_BIND 2341 2348 ATP (Potential).
 REGION 1 1289 Stem (By similarity).
 REGION 1290 1511 AAA 1 (By similarity).
 REGION 1571 1802 AAA 2 (By similarity).
 REGION 1938 2189 AAA 3 (By similarity).
 REGION 2302 2555 AAA 4 (By similarity).
 REGION 2572 2873 Stalk (By similarity).
 REGION 2954 3184 AAA 5 (By similarity).
 REGION 3397 3620 AAA 6 (By similarity).  
Keyword
 Alternative splicing; ATP-binding; Cell projection; Cilium; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Dynein; Microtubule; Motor protein; Nucleotide-binding; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4024 AA 
Protein Sequence
MSSEQDKSAS KEKSKKPVRF LPQLSMEKLA SKEKFKAPAR ALPQLSMVST KPHWQQAAPS 60
FHLSVKQDDE SPEPFSVKNE QSHAEYMERF GKKGKLPHQV DDSYVGPSTS KSKGKSPHKE 120
RENFRSTLVN VIMQQDADLD SAVPDGSTIP KPTASAIEKD ILRYYYYIHH GIDTDHVAPM 180
EDSWLEHVLD LVPQHLKVFT DSIVTLSDEM REDYLLSVRK SIVDFVLKDP REKGDDKKTD 240
ELPAHRAEME ILPKPWRKSF LAASSYIRDH LNAMNPTMLA VLDLWHTNFK KLRLVDIKEF 300
HNCQDALELS SFQNIIMRHM DSAKETLLKM WFPEVQNIYY QGNKKKQLPT GDSSAKLESF 360
FNCAAALMTL QLQDLTLVSM QDFTDLIAQP PDSVRAFEHP GFIMRLILDN DTIKFEPELS 420
DYIDIFLNVY DVMIKAVSFV PRVETKLYSK WESKSKPTTL KPIILNEIVD AHKEKIKEVI 480
MKESVAPTEH LRLYDKYDFL ITRKAERDVD NFLAENHSYE KIIDEICKYQ KLIEEIQYTS 540
IKTIRLGMFE MHCEELIRAL VKRADIICGK LLAKMFRDHQ EVNTRLCDEF ERIAEKALST 600
PPNTAELMEM KAYIQKVEVT DMIELEQRLV DSKNCLAFLI EYVNFSPADM RLNNSVFQWY 660
GRMGEIFEEH RKIIKEKIEQ YQEGLKLRCE RFVEELESYA KQSEEFYSFG DLQDVQRYLK 720
KAQILNGKLD LAADKIEQFN AEEEAFGWLP SVYPQRKKIQ DGLNPYLRLY ETAVEFSSNY 780
RAWTEGPYHK VNPDQVEADI GNYWRGLYKL EKTFHDSPYA LAMTKKVRSK VEDFKQHIPL 840
IQVICNPGLR PRHWEAMSAI VGYPLQPSDD STVSSFLDMN LEPYIDRFEG ISEAASKEYS 900
LEKAMEKMIT EWDAVEFVIH SYRETGTFIL ASVDEIQMLL DDHIIKTQTM RGSPFIKPYE 960
KQMREWEGKL LLLQEILDEW LKVQATWLYL EPIFSSPDIM SQMPEEGRRF TAVDKTWRDI 1020
MRSVMQDKHV LTVVTIDRML ERLKKSNELL ELILKGLNEY LEKKRLFFPR FFFLSNDELL 1080
EILSETKDPT RVQPHLKKCF EGIAKVEFTE TLDITHMKSS EGEVVELIEI ISTAKARGQV 1140
EKWLVELERV MINSIHKVTG DATFAYTKYE RINWVRDWPG QTVLCVSQIF WTKEVQTAIP 1200
MGIKALEQYL KTCNRQIDDI VTLVRGKLSM QNRVTLGALV VLDVHARDVL SSLVKKNISD 1260
DSDFEWLSQL RYYWQENHLE TKMINAGLRY GYEYLGNSPR LVITPLTDRC YRTLFGALHL 1320
HLGGAPEGPA GTGKTETTKD LAKAVAKQCV VFNCSDGLDY LALGKFFKGL LSCGAWACFD 1380
EFNRIDLEVL SVVAQQILTI QRGINAGADI LMFEGTELKL DPTCAVFITM NPGYAGRSEL 1440
PDNLKALFRT VAMMVPDYAM IAEIVLYSCG FVTARPLSVK IVATYRLCSE QLSSQHHYDY 1500
GMRAVKSVLT AAGNLKLKYP NENEEILLLR SIIDVNLPKF LSHDLPLFEG ITSDLFPGVK 1560
LPKPDYNDLL AAIKDNCASM NLQMTAFFSE KILQVYEMMI VRHGFMIVGE PFGGKTSAYR 1620
VLAGALNDIC EKGLMEENKV QITVLNPKSV TMGQLYGQFD SVSHEWSDGV LAVSFRAFAS 1680
SVTPDRKWLI FDGPVDAVWI ENMNTVLDDN KKLCLMSGEI IQMSPQMNLI FEPMDLEVAS 1740
PATVSRCGMI YMEPHMLGWR PLMLSWVNLL PASVSVIQKE FIMGLFDRMV PVSVEFIRKH 1800
TKELSPTSDT NLVRSLMNLI DCFMDDFADE VKLKERNDRE TYSLLEGIFL FSLIWSVGAS 1860
CTDDDRLKFN KILRELMESP ISDRTRNTFK LQSGTEQTSS KALTVPFPEK GTIYDYQFVT 1920
EGIGKWEPWI KKLKEAPPIP KDVMFNEIIV PTLDTIRYSA LMELLTTHQK PSIFVGPTGT 1980
GKSVYITNFL LNQLNKEIYK PLLINFSAQT TAAQTQNIVM SKLDKRRKGV FGPPLGKRMV 2040
VFVDDVNMPA REVYGAQPPI ELLRQWLDHW NWYDLKDCSM IKLVDIQIMC AMGPPGGGRN 2100
PVTPRYMRHF NIITINEFSD KSMYTIFSRI LTWHLEICYK FPDEFLDLTT QIVNGTMTLY 2160
KEAMKNLLPT PAKSHYLFNL RDFSRVIQGV CLSRPETTET TEVIKRLWVH EVLRVYYDRL 2220
LDNTDRSWLI NYIQEILRNY MYEDFHELFQ RLDFDNDGMV EADDLRSLMF CDFHDPKRED 2280
TNYREIADVD NLRMIVEIHL EEYNNISKKP MNLVLFRFAI EHISRISRIL KQPRSHALLV 2340
GVGGSGRQSV TRLAAHMADY SVFQVEISKG YDTTEWHEDL KVILRKCAEG EMQGVFLFTD 2400
TQIKEESFLE DVSNLLNAGE IPNLFALDEK QEICDKMRQL DRQRDKTKQT DGSPIALFNM 2460
FIDHCRSQLH VVLAMSPIGD AFRNRLRKFP ALVNCCTIDW FQSWPEDALQ AVASRFLEEI 2520
EMSEEIRDGC IDMCKSFHTS TIDLSKSFFV ELQRYNYVTP TSYLELISTF KLLLEKKRSE 2580
VMKMKKRYEV GLEKLDSASS QVATMQMELE ALHPQLKVAS KEVDEMMIMI EKESVEVAKT 2640
EKIVKADETI ANEQAMASKA IKDECDADLA GALPILESAL AALDTLTAQD ITVVKSMKSP 2700
PAGVKLVMEA ICILKGIKAD KIPDPTGSGK KIEDFWGPAK RLLGDMRFLQ SLHEYDKDNI 2760
PPAYMNIIRK NYIPNPDFVP EKIRNASTAA EGLCKWVIAM DSYDKVAKIV APKKIKLAAA 2820
EGELKIAMDG LRKKQAALKE VQDKLARLQD TLELNKQKKA DLENQVDLCS KKLERAEQLI 2880
GGLGGEKTRW SHTALELGQL YINLTGDILI SSGVVAYLGA FTSTYRQNQT KEWTTLCKGR 2940
DIPCSDDCSL MGTLGEAVTI RTWNIAGLPS DSFSIDNGII IMNARRWPLM IDPQSQANKW 3000
IKNMEKANSL YVIKLSEPDY VRTLENCIQF GTPVLLENVG EELDPILEPL LLKQTFKQGG 3060
STCIRLGDST IEYAPDFRFY ITTKLRNPHY LPETSVKVTL LNFMITPEGM QDQLLGIVVA 3120
QERPDLEEEK QALILQGAEN KRQLKEIEDK ILEVLSSSEG NILEDETAIK ILSSSKALAN 3180
EISQKQEVAE ETEKKIDTTR MGYRPIAIHS SILFFSLADL ANIEPMYQYS LTWFINLFIL 3240
SIENSEKSEI LAKRLQILKD HFTYSLYVNV CRSLFEKDKL LFSFCLTINL LLHERAINKA 3300
EWRFLLTGGI GLDNPYANLC TWLPQKSWDE ICRLDDLPAF KTIRREFMRL KDGWKKVYDS 3360
LEPHHEVFPE EWEDKANEFQ RMLIIRCLRP DKVIPMLQEF IINRLGRAFI EPPPFDLAKA 3420
FGDSNCCAPL IFVLSPGADP MAALLKFADD QGYGGSKLSS LSLGQGQGPI AMKMLEKAVK 3480
EGTWVVLQNC HLATSWMPTL EKVCEELSPE STHPDFRMWL TSYPSPNFPV SVLQNGVKMT 3540
NEAPKGLRAN IIRSYLMDPI SDPEFFGSCK KPEEFKKLLY GLCFFHALVQ ERRKFGPLGW 3600
NIPYEFNETD LRISVQQLHM FLNQYEELPY EALRYMTGEC NYGGRVTDDW DRRTLRSILN 3660
KFFNPELVEN SDYKFDSSGI YFVPPSGDHK SYIEYTKTLP LTPAPEIFGM NANADITKDQ 3720
SETQLLFDNI LLTQSRSAGA GAKSSDEVVN EVASDILGKL PNNFDIEAAM RRYPTTYTQS 3780
MNTVLVQEMG RFNKLLKTIR DSCVNIQKAI KGLAVMSTDL EEVVSSILNV KIPEMWMGKS 3840
YPSLKPLGSY VNDFLARLKF LQQWYEVGPP PVFWLSGFFF TQAFLTGAQQ NYARKYTIPI 3900
DLLGFDYEVM EDKEYKHPPE DGVFIHGLFL DGASWNRKIK KLAESHPKIL YDTVPVMWLK 3960
PCKRADIPKR PSYVAPLYKT SERRGVLSTT GHSTNFVIAM TLPSDQPKEH WIGRGVALLC 4020
QLNS 4024 
Gene Ontology
 GO:0005858; C:axonemal dynein complex; NAS:UniProtKB.
 GO:0005929; C:cilium; IDA:MGI.
 GO:0035085; C:cilium axoneme; IEA:UniProtKB-SubCell.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003777; F:microtubule motor activity; NAS:UniProtKB.
 GO:0006200; P:ATP catabolic process; IEA:GOC.
 GO:0001539; P:ciliary or flagellar motility; NAS:UniProtKB.
 GO:0007018; P:microtubule-based movement; IEA:InterPro. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR026983; DHC_fam.
 IPR026979; DNAH7.
 IPR024743; Dynein_HC_stalk.
 IPR024317; Dynein_heavy_chain_D4_dom.
 IPR004273; Dynein_heavy_dom.
 IPR013602; Dynein_heavy_dom-2.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF12780; AAA_8
 PF08393; DHC_N2
 PF03028; Dynein_heavy
 PF12777; MT 
SMART
 SM00382; AAA 
PROSITE
  
PRINTS