UniProt Accession

 EFTS_ECOLI; P0A6P1; P02997 

Genbank Protein ID

 V00343; D13334; U00096; AP009048; U70214 

Genbank Nucleotide ID

 CAA23632.1; BAA02597.1; AAC73281.1; BAB96746.1; AAB08599.1 

Protein Name

 Elongation factor Ts 

Protein Synonyms/Alias

 EF-Ts 

Gene Name

 tsf 

Gene Synonyms/Alias

 b0170; JW0165 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
24	GAGMMDCKKALTEAN	acetylation	[1]
48	MRKSGAIKAAKKAGN	acetylation	[1]
51	SGAIKAAKKAGNVAA	acetylation	[1]
52	GAIKAAKKAGNVAAD	acetylation	[1]
95	GFQAFADKVLDAAVA	acetylation	[1, 2, 3]
104	LDAAVAGKITDVEVL	acetylation	[1]
112	ITDVEVLKAQFEEER	acetylation	[1]
125	ERVALVAKIGENINI	acetylation	[1]
159	IGVLVAAKGADEELV	acetylation	[1]
167	GADEELVKHIAMHVA	acetylation	[1]
177	AMHVAASKPEFIKPE	acetylation	[1, 3]
182	ASKPEFIKPEDVSAE	acetylation	[1]
207	DIAMQSGKPKEIAEK	acetylation	[1]
209	AMQSGKPKEIAEKMV	acetylation	[1]
214	KPKEIAEKMVEGRMK	acetylation	[1]
221	KMVEGRMKKFTGEVS	acetylation	[1]
222	MVEGRMKKFTGEVSL	acetylation	[1]
240	PFVMEPSKTVGQLLK	acetylation	[1]
247	KTVGQLLKEHNAEVT	acetylation	[1, 2, 3]
267	EVGEGIEKVETDFAA	acetylation	[1, 3]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. 

Sequence Annotation

 REGION 80 83 Involved in Mg(2+) ion dislocation from  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 283 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0003746; F:translation elongation factor activity; IEA:HAMAP.
 GO:0008270; F:zinc ion binding; IDA:EcoliWiki. 

Interpro

 IPR001816; Transl_elong_EFTs/EF1B.
 IPR014039; Transl_elong_EFTs/EF1B_dimer.
 IPR018101; Transl_elong_Ts_CS.
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N. 

Pfam

 PF00889; EF_TS
 PF00627; UBA 

SMART

  

PROSITE

 PS01126; EF_TS_1
 PS01127; EF_TS_2 

PRINTS