CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012241
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Low-density lipoprotein receptor-related protein 8 
Protein Synonyms/Alias
 LRP-8; Apolipoprotein E receptor 2 
Gene Name
 LRP8 
Gene Synonyms/Alias
 APOER2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
868FDNPVYRKTTEEEDEubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. 
Sequence Annotation
 DOMAIN 46 82 LDL-receptor class A 1.
 DOMAIN 85 123 LDL-receptor class A 2.
 DOMAIN 126 164 LDL-receptor class A 3.
 DOMAIN 166 202 LDL-receptor class A 4.
 DOMAIN 205 246 LDL-receptor class A 5.
 DOMAIN 258 295 LDL-receptor class A 6.
 DOMAIN 298 334 LDL-receptor class A 7.
 DOMAIN 336 375 EGF-like 1.
 DOMAIN 376 415 EGF-like 2; calcium-binding (Potential).
 REPEAT 462 508 LDL-receptor class B 1.
 REPEAT 509 551 LDL-receptor class B 2.
 REPEAT 552 595 LDL-receptor class B 3.
 REPEAT 596 639 LDL-receptor class B 4.
 REPEAT 640 681 LDL-receptor class B 5.
 REGION 740 798 Clustered O-linked oligosaccharides.
 CARBOHYD 176 176 N-linked (GlcNAc...) (Potential).
 CARBOHYD 441 441 N-linked (GlcNAc...) (Potential).
 CARBOHYD 518 518 N-linked (GlcNAc...) (Potential).
 CARBOHYD 538 538 N-linked (GlcNAc...) (Potential).
 CARBOHYD 772 772 N-linked (GlcNAc...) (Potential).
 CARBOHYD 807 807 N-linked (GlcNAc...) (Potential).
 DISULFID 47 59 By similarity.
 DISULFID 54 72 By similarity.
 DISULFID 66 81 By similarity.
 DISULFID 86 98 By similarity.
 DISULFID 93 111 By similarity.
 DISULFID 105 122 By similarity.
 DISULFID 127 141 By similarity.
 DISULFID 134 154 By similarity.
 DISULFID 148 163 By similarity.
 DISULFID 167 179 By similarity.
 DISULFID 174 192 By similarity.
 DISULFID 186 201 By similarity.
 DISULFID 206 221 By similarity.
 DISULFID 213 234 By similarity.
 DISULFID 228 245 By similarity.
 DISULFID 259 272 By similarity.
 DISULFID 267 285 By similarity.
 DISULFID 279 294 By similarity.
 DISULFID 299 311 By similarity.
 DISULFID 306 324 By similarity.
 DISULFID 318 333 By similarity.
 DISULFID 340 351 By similarity.
 DISULFID 347 360 By similarity.
 DISULFID 362 374 By similarity.
 DISULFID 380 390 By similarity.
 DISULFID 386 399 By similarity.
 DISULFID 401 414 By similarity.  
Keyword
 3D-structure; Alternative splicing; Calcium; Cell membrane; Complete proteome; Disulfide bond; EGF-like domain; Endocytosis; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Secreted; Signal; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 963 AA 
Protein Sequence
MGLPEPGPLR LLALLLLLLL LLLLQLQHLA AAAADPLLGG QGPAKDCEKD QFQCRNERCI 60
PSVWRCDEDD DCLDHSDEDD CPKKTCADSD FTCDNGHCIH ERWKCDGEEE CPDGSDESEA 120
TCTKQVCPAE KLSCGPTSHK CVPASWRCDG EKDCEGGADE AGCATLCAPH EFQCGNRSCL 180
AAVFVCDGDD DCGDGSDERG CADPACGPRE FRCGGDGGGA CIPERWVCDR QFDCEDRSDE 240
AAELCGRPGP GATSAPAACA TASQFACRSG ECVHLGWRCD GDRDCKDKSD EADCPLGTCR 300
GDEFQCGDGT CVLAIKHCNQ EQDCPDGSDE AGCLQGLNEC LHNNGGCSHI CTDLKIGFEC 360
TCPAGFQLLD QKTCGDIDEC KDPDACSQIC VNYKGYFKCE CYPGYEMDLL TKNCKAAAGK 420
SPSLIFTNRH EVRRIDLVKR NYSRLIPMLK NVVALDVEVA TNRIYWCDLS YRKIYSAYMD 480
KASDPKEQEV LIDEQLHSPE GLAVDWVHKH IYWTDSGNKT ISVATVDGGR RRTLFSRNLS 540
EPRAIAVDPL RGFMYWSDWG DQAKIEKSGL NGVDRQTLVS DNIEWPNGIT LDLLSQRLYW 600
VDSKLHQLSS IDFSGGNRKT LISSTDFLSH PFGIAVFEDK VFWTDLENEA IFSANRLNGL 660
EISILAENLN NPHDIVIFHE LKQPRAPDAC ELSVQPNGGC EYLCLPAPQI SSHSPKYTCA 720
CPDTMWLGPD MKRCYRAPQS TSTTTLASTM TRTVPATTRA PGTTVHRSTY QNHSTETPSL 780
TAAVPSSVSV PRAPSISPST LSPATSNHSQ HYANEDSKMG STVTAAVIGI IVPIVVIALL 840
CMSGYLIWRN WKRKNTKSMN FDNPVYRKTT EEEDEDELHI GRTAQIGHVY PAAISSFDRP 900
LWAEPCLGET REPEDPAPAL KELFVLPGEP RSQLHQLPKN PLSELPVVKS KRVALSLEDD 960
GLP 963 
Gene Ontology
 GO:0005901; C:caveola; IDA:BHF-UCL.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IC:UniProtKB.
 GO:0034185; F:apolipoprotein binding; IC:BHF-UCL.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0038025; F:reelin receptor activity; ISS:BHF-UCL.
 GO:0030229; F:very-low-density lipoprotein particle receptor activity; IDA:BHF-UCL.
 GO:0021541; P:ammon gyrus development; ISS:BHF-UCL.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; NAS:UniProtKB.
 GO:0021819; P:layer formation in cerebral cortex; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:BHF-UCL.
 GO:0007603; P:phototransduction, visible light; TAS:Reactome.
 GO:0032793; P:positive regulation of CREB transcription factor activity; ISS:BHF-UCL.
 GO:1900006; P:positive regulation of dendrite development; ISS:BHF-UCL.
 GO:0061003; P:positive regulation of dendritic spine morphogenesis; ISS:BHF-UCL.
 GO:0061098; P:positive regulation of protein tyrosine kinase activity; ISS:BHF-UCL.
 GO:0006508; P:proteolysis; NAS:UniProtKB.
 GO:0050804; P:regulation of synaptic transmission; ISS:BHF-UCL.
 GO:0001523; P:retinoid metabolic process; TAS:Reactome. 
Interpro
 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR026823; cEGF.
 IPR000742; EG-like_dom.
 IPR001881; EGF-like_Ca-bd.
 IPR013032; EGF-like_CS.
 IPR000152; EGF-type_Asp/Asn_hydroxyl_site.
 IPR018097; EGF_Ca-bd_CS.
 IPR009030; Growth_fac_rcpt_N_dom.
 IPR023415; LDLR_class-A_CS.
 IPR000033; LDLR_classB_rpt.
 IPR002172; LDrepeatLR_classA_rpt. 
Pfam
 PF12662; cEGF
 PF00057; Ldl_recept_a
 PF00058; Ldl_recept_b 
SMART
 SM00181; EGF
 SM00179; EGF_CA
 SM00192; LDLa
 SM00135; LY 
PROSITE
 PS00010; ASX_HYDROXYL
 PS00022; EGF_1
 PS01186; EGF_2
 PS50026; EGF_3
 PS01187; EGF_CA
 PS01209; LDLRA_1
 PS50068; LDLRA_2
 PS51120; LDLRB 
PRINTS