CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006346
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translational activator GCN1 
Protein Synonyms/Alias
  
Gene Name
 GCN1 
Gene Synonyms/Alias
 YGL195W; G1318 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
802LVNEQLAKESAVRSHubiquitination[1]
1446HTTGYGVKKLIPVAVmethylation[2]
1659DTLSDESKSGDRLGSubiquitination[1]
2056VAGSALYKRLSIIINacetylation[3]
2480NGTIAAGKLLLLEGEubiquitination[4]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 Acts as a translation activator that mediates translational control by regulating GCN2 kinase activity. Translational activator of GCN4. May be involved in sensing charged tRNA and stimulating the kinase activity of GCN2 in amino acid-starved cells. Required in vivo for the phosphorylation of eIF-2-alpha on 'Ser-52' by the protein kinase GCN2. 
Sequence Annotation
 REPEAT 932 970 HEAT 1.
 REPEAT 1030 1067 HEAT 2.
 REPEAT 1099 1138 HEAT 3.
 REPEAT 1243 1281 HEAT 4.
 REPEAT 1284 1321 HEAT 5.
 REPEAT 1405 1442 HEAT 6.
 REPEAT 1484 1521 HEAT 7.
 REPEAT 1523 1559 HEAT 8.
 REPEAT 1561 1598 HEAT 9.
 REPEAT 1603 1640 HEAT 10.
 REPEAT 1641 1679 HEAT 11.
 REPEAT 1721 1758 HEAT 12.
 REPEAT 1760 1796 HEAT 13.
 REPEAT 1862 1903 HEAT 14.
 REPEAT 1905 1942 HEAT 15.
 REPEAT 1947 1984 HEAT 16.
 REPEAT 1985 2024 HEAT 17.
 REPEAT 2097 2134 HEAT 18.
 REPEAT 2290 2328 HEAT 19.
 REPEAT 2347 2384 HEAT 20.  
Keyword
 Activator; Complete proteome; Reference proteome; Repeat; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2672 AA 
Protein Sequence
MTAILNWEDI SPVLEKGTRE SHVSKRVPFL QDISQLVRQE TLEKPQLSEI AFVLLNTFTI 60
YEDNRSKSLV TSILLDILNL EPCLLENFIR FISDVVISNP ATKAVADYLN LLDWINSFLI 120
FVSHNSNLFE EYIPKLLVAH SYATFGVETI LDNQEEGKKS QDKQNQHRKR IRYCIFQTTV 180
KAFLKCLKDN DDSISFMKIS IKTVLESYSK LKITSVGVVM IMGALTQAAL QLLSRQPALH 240
SVLKENSAEK YCEYLGKEVF LGKNPPSSFC LEIGLKPFLK EFVSQELFIK FFIPNIEKAV 300
LRSPEVGFSI LSELYAGVSP EKVNLLNAFA SSKLINQYFS SFKSSKEVVR SVSLQSMIIL 360
LRKISNTDTT LEDLTKLIDE IFKNIKSNLN ADYKSLISKI LIEIPLTHYE VSEKICKGLS 420
PYIGKEGNEA ALTLMLNAFF VHYFSLGKPI EDLDKIISAG FADKKPALKK CWFAAFLNNS 480
NAASEEVILN FIDGCLEFVK DSIIHYQTHG HACILASIEF TNKILALDNT ELNDRVMQLI 540
ETLPENSSIG DAILTAALST ELSIENRIHA VNLLQELFYK KPEFIGFSVI DAIERRMRVQ 600
ELIPQQNTSF KYVTSVLLAI TSELPDKEAS IKVLINALVI AQWNIFNIKN GWAGLVLRAR 660
LDPAEVVKEH ASVIMEKILE ITGSCEWIDT IYGACGLQAA AYAAFIQPNE FTPILCKTIE 720
ADLTADDFSR LSEEDFEIFA GEEGVLVVDV LEESMNKKLS NKNSKEYETL MWEQKIRKEQ 780
AKKNVKKLSK EEQELVNEQL AKESAVRSHV SEISTRLKRG IRLVSELSKA ACLVQNGIAT 840
WFPLAVTKLL YLCSEPNISK LTEDVNNVFL QLSQNVSERL GNIRLFLGLA TLRVHNANGI 900
SQDYLQEPLV ELLTRVLFRI KFVSNQAAID SISLTYILPL LINVLEKGKA IALKNADKPV 960
VKAEFVEEDE EEEHLLLAME IISVHAEAFE DPSIPRISIV EVLLSLLSLP SKAKIAKDCF 1020
NALCQSISVA PNQEDLDMIL SNLLSPNQFV RSTILETLDN EFELEPFMKY SPEVFICRFD 1080
SDPSNREIAD FIWEFNKFVV NDELLKSLFP LFNQDDSGLR LFAANAYAFG AVSLFTSEEN 1140
SSKDYLNDLL NFYKEKAKPL EPILDQFGLV LVSASEQKDP WQGRSTVAIT LKIMAKAFSA 1200
EDDTVVNIIK FLVDDGGLVD REPIVRQEMK EAGVELITLH GSQNSKDLIP IFEEALSSST 1260
DSALKENVII LYGTLARHLQ QSDARIHTII ERLLSTLDTP SADIQQAVSA CIAPLVFQFK 1320
QKVGDYLGIL MEKLLNPTVA SSMRKGAAWG IAGLVKGYGI SALSEFDIIR NLIEAAEDKK 1380
EPKRRESVGF CFQYLSESLG KFFEPYVIEI LPNILKNLGD AVPEVRDATA RATKAIMAHT 1440
TGYGVKKLIP VAVSNLDEIA WRTKRGSVQL LGNMAYLDPT QLSASLSTIV PEIVGVLNDS 1500
HKEVRKAADE SLKRFGEVIR NPEIQKLVPV LLQAIGDPTK YTEEALDSLI QTQFVHYIDG 1560
PSLALIIHII HRGMHDRSAN IKRKACKIVG NMAILVDTKD LIPYLQQLID EVEIAMVDPV 1620
PNTRATAARA LGALVERLGE EQFPDLIPRL LDTLSDESKS GDRLGSAQAL AEVISGLGLT 1680
KLDEMLPTIL AGVTNFRAYI REGFMPLLLF LPVCFGSQFA PYINQIIQPI LSGLADNDEN 1740
IRDTALKAGK LIVKNYATKA VDLLLPELER GMFDENDRIR LSSVQLTGEL LFQVTGISSR 1800
NEFSEEDGDH NGEFSGKLVD VLGQDRRDRI LAALFVCRND TSGIVRATTV DIWKALVPNT 1860
PRAVKEILPT LTGMIVTHLA SSSNVLRNIA AQTLGDLVRR VGGNALSQLL PSLEESLIET 1920
SNSDSRQGVC IALYELIESA STETISQFQS TIVNIIRTAL IDESATVREA AALSFDVFQD 1980
VVGKTAVDEV LPYLLHMLES SDNSDFALLG LQEIMSKKSD VIFPILIPTL LAPPIDAFRA 2040
SALGSLAEVA GSALYKRLSI IINALVDAII GTSEDESTKG ALELALDRVF LSVNDDEGLH 2100
PLLQQIMSLL KSDNIEKRIA VLERLPNFFD KTVLDFDVYI PNFVSHAILS LDDEDQRVVN 2160
GNFNALSTLL KKVDKPTLEK LVKPAKQSLA LTGRQGQDVA AFKLPRGPNC VLPIFLHGLM 2220
YGSNDEREES ALAIADVVSK TPAANLKPFV SVITGPLIRV VGERFSSDIK AAILFALNVL 2280
FIKIPMFLRP FIPQLQRTFV KSLSDATNET LRLRAAKALG ALIEHQPRVD PLVIELVTGA 2340
KQATDEGVKT AMLKALLEVI MKAGSKLNEN SKTNIVNLVE EEMLGSNDKL AVAYAKLIGS 2400
LSEILSNDEA HKILQDKVLN ADLDGETGKF AILTLNSFLK DAPTHIFNTG LIDEFVSYIL 2460
NAIRSPDVYF GENGTIAAGK LLLLEGEKRS PFVKKDAAEP FKIGDENINL LINELSKAVL 2520
QPASNSTDVR RLALVVIRTL ARFKFDECIK QYFDVVGPSV FSCLRDPVIP IKLAAEKAYL 2580
ALFKLVEEDD MHTFNEWFAK ISDRGNSIET VTGTTIQLRS VGDYTKRVGK RLANVERERI 2640
AAGGDAETMF SDRFEDEREI WAVGGVELTT DI 2672 
Gene Ontology
 GO:0022626; C:cytosolic ribosome; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0006448; P:regulation of translational elongation; IPI:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR022716; DUF3554.
 IPR026827; ECM29/GCN1.
 IPR021133; HEAT_type_2. 
Pfam
 PF12074; DUF3554 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS