CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008450
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cyclin-H 
Protein Synonyms/Alias
 MO15-associated protein; p34; p37 
Gene Name
 CCNH 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MYHNSSQKRHWTFSSubiquitination[1]
39CKAVANGKVLPNDPVubiquitination[1]
72LEFCSVFKPAMPRSVubiquitination[1, 2]
253SQLLDIMKSMRNLVKubiquitination[1]
306DDDYVSKKSKHEEEEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. 
Sequence Annotation
 MOD_RES 5 5 Phosphoserine; by CDK8.
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 304 304 Phosphoserine; by CDK8.
 MOD_RES 315 315 Phosphothreonine.
 MOD_RES 322 322 Phosphoserine.  
Keyword
 3D-structure; Cell cycle; Complete proteome; Cyclin; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 323 AA 
Protein Sequence
MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY 60
EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV 120
SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI 180
LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE 240
NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD 300
DYVSKKSKHE EEEWTDDDLV ESL 323 
Gene Ontology
 GO:0019907; C:cyclin-dependent protein kinase activating kinase holoenzyme complex; IDA:UniProtKB.
 GO:0005675; C:holo TFIIH complex; IDA:UniProtKB.
 GO:0070985; C:TFIIK complex; IEA:InterPro.
 GO:0016538; F:cyclin-dependent protein serine/threonine kinase regulator activity; IEA:InterPro.
 GO:0016301; F:kinase activity; IEA:Compara.
 GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0016310; P:phosphorylation; IEA:GOC.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; IEA:InterPro.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR013763; Cyclin-like.
 IPR015429; Cyclin_C/H/T/L.
 IPR006671; Cyclin_N.
 IPR023598; CyclinC.
 IPR027081; CyclinH/Ccl1. 
Pfam
 PF00134; Cyclin_N 
SMART
 SM00385; CYCLIN 
PROSITE
 PS00292; CYCLINS 
PRINTS