CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003975
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Arginine--tRNA ligase 
Protein Synonyms/Alias
 Arginyl-tRNA synthetase; ArgRS 
Gene Name
 argS 
Gene Synonyms/Alias
 b1876; JW1865 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
112LGVATPEKQTIVVDYacetylation[1]
126YSAPNVAKEMHVGHLacetylation[1]
152TLEFLGHKVIRANHVacetylation[1]
197EGFYRDAKKHYDEDEacetylation[1]
216RARNYVVKLQSGDEYacetylation[1]
230YFREMWRKLVDITMTacetylation[1]
295FLDEFKNKEGEPMGVacetylation[1]
306PMGVIIQKKDGGYLYacetylation[1]
307MGVIIQKKDGGYLYTacetylation[1]
322TTDIACAKYRYETLHacetylation[1]
375MFGMMLGKDGKPFKTacetylation[1]
389TRAGGTVKLADLLDEacetylation[1]
408ARRLVAEKNPDMPADacetylation[1]
419MPADELEKLANAVGIacetylation[1]
473RVLSVFRKAEIDEEQacetylation[1]
563QLTAKTLKLGLDTLGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 MOTIF 122 132 "HIGH" region.  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 577 AA 
Protein Sequence
MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK LGMAPRQLAE 60
QVLTHLDLNG IASKVEIAGP GFINIFLDPA FLAEHVQQAL ASDRLGVATP EKQTIVVDYS 120
APNVAKEMHV GHLRSTIIGD AAVRTLEFLG HKVIRANHVG DWGTQFGMLI AWLEKQQQEN 180
AGEMELADLE GFYRDAKKHY DEDEEFAERA RNYVVKLQSG DEYFREMWRK LVDITMTQNQ 240
ITYDRLNVTL TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EFKNKEGEPM 300
GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA IVRKAGYVPE 360
SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER ARRLVAEKNP DMPADELEKL 420
ANAVGIGAVK YADLSKNRTT DYIFDWDNML AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ 480
LAAAPVIIRE DREAQLAARL LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL 540
SAENEEVRNS RLKLAQLTAK TLKLGLDTLG IETVERM 577 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004814; F:arginine-tRNA ligase activity; IEA:HAMAP.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0006420; P:arginyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR001278; Arg-tRNA-ligase_Ia.
 IPR015945; Arg-tRNA-synth_Ia_core.
 IPR005148; Arg-tRNA-synth_N.
 IPR008909; DALR_anticod-bd.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF03485; Arg_tRNA_synt_N
 PF05746; DALR_1
 PF00750; tRNA-synt_1d 
SMART
 SM01016; Arg_tRNA_synt_N
 SM00836; DALR_1 
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR01038; TRNASYNTHARG.