UniProt Accession

 FXRD2_HUMAN; Q8IWF2; B2RDI4; Q8N378; Q96BD1; Q9H5L5; Q9H6M8 

Genbank Protein ID

 CR456444; AK025714; AK026975; AK315555; AL022313; CH471095; BC015726; BC027716; BC040351 

Genbank Nucleotide ID

 CAG30330.1; BAB15227.1; BAB15610.1; BAG37931.1; CAI19228.1; EAW60106.1; AAH15726.1; AAH27716.1; AAH40351.1 

Protein Name

 FAD-dependent oxidoreductase domain-containing protein 2 

Protein Synonyms/Alias

 Endoplasmic reticulum flavoprotein associated with degradation 

Gene Name

 FOXRED2 

Gene Synonyms/Alias

 ERFAD 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
77	TRYPRHRKLISINKR	ubiquitination	[1]
83	RKLISINKRYTGKAN	ubiquitination	[1]
88	INKRYTGKANAEFNL	ubiquitination	[1, 2, 3, 4]
153	IAHVTLDKDRQAWNG	ubiquitination	[1]
290	LTDLAILKDSKGKFH	ubiquitination	[1, 3]
295	ILKDSKGKFHVTPKF	ubiquitination	[1]
350	FDFSIFNKSLRLNSG	ubiquitination	[3]
362	NSGNAFGKKYPLIRA	ubiquitination	[2, 3]
363	SGNAFGKKYPLIRAS	ubiquitination	[1]
374	IRASYESKGSRGLFI	ubiquitination	[2]
393	SHSVDYRKSAGGFIH	ubiquitination	[1]
508	RNFSGPDKDVFFDDR	ubiquitination	[1]
610	LFALTRQKLPPFCQQ	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Probable flavoprotein which may function in endoplasmic reticulum associated degradation (ERAD). May bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation. 

Sequence Annotation

 MOTIF 681 684 Prevents secretion from ER (Potential).
 CARBOHYD 143 143 N-linked (GlcNAc...).  

Keyword

 Alternative splicing; Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Glycoprotein; Oxidoreductase; Polymorphism; Reference proteome; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 684 AA 

Protein Sequence

Gene Ontology

 GO:0005788; C:endoplasmic reticulum lumen; IDA:UniProtKB.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:UniProtKB.
 GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
 GO:0030433; P:ER-associated protein catabolic process; IMP:UniProtKB. 

Interpro

  

Pfam

  

SMART

  

PROSITE

 PS00014; ER_TARGET 

PRINTS