CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010474
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-isopropylmalate dehydratase large subunit 
Protein Synonyms/Alias
 Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; Superoxide-inducible protein 10; SOI10 
Gene Name
 leuC 
Gene Synonyms/Alias
 BSU28260 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
22IVKHGEGKPDLLYIDacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. 
Sequence Annotation
 METAL 347 347 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 407 407 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 410 410 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 472 AA 
Protein Sequence
MMPRTIIEKI WDQHIVKHGE GKPDLLYIDL HLIHEVTSPQ AFEGLRQKGR KVRRPQNTFA 60
TMDHNIPTVN RFEIKDEVAK RQVTALERNC EEFGVRLADL HSVDQGIVHV VGPELGLTLP 120
GKTIVCGDSH TSTHGAFGAL AFGIGTSEVE HVLSTQTLWQ QRPKTLEVRV DGTLQKGVTA 180
KDVILAVIGK YGVKFGTGYV IEYTGEVFRN MTMDERMTVC NMSIEAGARA GLIAPDEVTF 240
EYCKNRKYTP KGEEFDKAVE EWKALRTDPG AVYDKSIVLD GNKISPMVTW GINPGMVLPV 300
DSEVPAPESF SAEDDKKEAI RAYEYMGLTP HQKIEDIKVE HVFIGSCTNS RMTDLRQAAD 360
MIKGKKVADS VRAIVVPGSQ SVKLQAEKEG LDQIFLEAGF EWRESGCSMC LSMNNDVVPE 420
GERCASTSNR NFEGRQGKGA RTHLVSPAMA AMAAIHGHFV DVRKFYQEKT VV 472 
Gene Ontology
 GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:HAMAP.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009098; P:leucine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR004430; 3-IsopropMal_deHydase_lsu.
 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS. 
Pfam
 PF00330; Aconitase 
SMART
  
PROSITE
 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 
PRINTS
 PR00415; ACONITASE.