CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014402
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein RFP 
Protein Synonyms/Alias
 Ret finger protein; Tripartite motif-containing protein 27 
Gene Name
 Trim27 
Gene Synonyms/Alias
 Rfp 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
380WEVEVGDKAKWTIGVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Has a transcriptional repressor activity. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double- stranded DNA. E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation (By similarity). 
Sequence Annotation
 DOMAIN 298 492 B30.2/SPRY.
 ZN_FING 16 57 RING-type.
 ZN_FING 91 132 B box-type.  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; DNA-binding; Ligase; Metal-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MASGSVAECL QQETTCPVCL QYFVEPMMLD CGHNICCACL ARCWGAAETN VSCPQCRETF 60
PQRHMRPNRH LANVTQLVKQ LRTERPSGPG GEMGVCEKHR EPLKLYCEQD QMPICVVCDR 120
SREHRGHSVL PLEEAVEGFK EQIQNRLDHL RRVKDLKKRR RAQGEQARAE LLSLTQMERE 180
KIVWEFEQLY HSLKEHEYRL LARLEELDLA IYNSINGAIT QFSCNISHLS GLIAQLEEKQ 240
QQPTRELLQD IGDTLSRAER IRIPEPWITP PDLQEKIHIF AQKCLFLTES LKQFTEKMQS 300
DMEKIQELRE AQLYSVDVTL DPDTAYPSLI LSDNLRQVRY SYLQQDLPDN PERFNLFPCV 360
LGSPCFIAGR HYWEVEVGDK AKWTIGVCED SVCRKGGVTS APQNGFWAVS LWYGKEYWAL 420
TSPMTALPLR TPLQRVGIFL DYDAGEVSFY NVTERCHTFT FSHATFCGPV RPYFSLSYSG 480
GKSAAPLIIC PMSGIDGFSG HVGNHGHSME TSP 513 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0031965; C:nuclear membrane; ISO:MGI.
 GO:0005654; C:nucleoplasm; ISO:MGI.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0072643; P:interferon-gamma secretion; ISS:UniProtKB.
 GO:0002820; P:negative regulation of adaptive immune response; ISS:UniProtKB.
 GO:0090281; P:negative regulation of calcium ion import; ISS:UniProtKB.
 GO:0045814; P:negative regulation of gene expression, epigenetic; ISO:MGI.
 GO:1900041; P:negative regulation of interleukin-2 secretion; ISS:UniProtKB.
 GO:0006469; P:negative regulation of protein kinase activity; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IGI:MGI.
 GO:0032720; P:negative regulation of tumor necrosis factor production; ISS:UniProtKB.
 GO:0070206; P:protein trimerization; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR003879; Butyrophylin.
 IPR008985; ConA-like_lec_gl_sf.
 IPR006574; PRY.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt.
 IPR000315; Znf_B-box.
 IPR020457; Znf_B-box_chordata.
 IPR018957; Znf_C3HC4_RING-type.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF00622; SPRY
 PF00643; zf-B_box
 PF00097; zf-C3HC4 
SMART
 SM00336; BBOX
 SM00589; PRY
 SM00184; RING
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS50119; ZF_BBOX
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS
 PR01406; BBOXZNFINGER.
 PR01407; BUTYPHLNCDUF.