CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014959
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase 4 regulatory subunit 3A 
Protein Synonyms/Alias
 SMEK homolog 1 
Gene Name
 SMEK1 
Gene Synonyms/Alias
 KIAA2010; PP4R3A; PPP4R3A; MSTP033 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
566KRKIIGLKDEFYNRYubiquitination[1, 2]
576FYNRYIMKSFLFEPVubiquitination[1, 2, 3]
585FLFEPVVKAFLNNGSubiquitination[1, 2, 3]
655RERQDNPKLDSMRSIacetylation[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. 
Sequence Annotation
 DOMAIN 1 100 WH1.
 MOD_RES 117 117 Phosphoserine.
 MOD_RES 655 655 N6-acetyllysine.
 MOD_RES 741 741 Phosphoserine.
 MOD_RES 771 771 Phosphoserine.
 MOD_RES 774 774 Phosphoserine.
 MOD_RES 777 777 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 833 AA 
Protein Sequence
MTDTRRRVKV YTLNEDRQWD DRGTGHVSSG YVERLKGMSL LVRAESDGSL LLESKINPNT 60
AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVDIT QDLVDESEEE 120
RFDDMSSPGL ELPSCELSRL EEIAELVASS LPSPLRREKL ALALENEGYI KKLLELFHVC 180
EDLENIEGLH HLYEIIKGIF LLNRTALFEV MFSEECIMDV IGCLEYDPAL SQPRKHREFL 240
TKTAKFKEVI PISDPELKQK IHQTYRVQYI QDMVLPTPSV FEENMLSTLH SFIFFNKVEI 300
VGMLQEDEKF LTDLFAQLTD EATDEEKRQE LVNFLKEFCA FSQTLQPQNR DAFFKTLSNM 360
GILPALEVIL GMDDTQVRSA ATDIFSYLVE YNPSMVREFV MQEAQQNDDV SKKLTEQKIT 420
SKDILLINLI IEHMICDTDP ELGGAVQLMG LLRTLVDPEN MLATANKTEK TEFLGFFYKH 480
CMHVLTAPLL ANTTEDKPSK DDFQTAQLLA LVLELLTFCV EHHTYHIKNY IINKDILRRV 540
LVLMASKHAF LALCALRFKR KIIGLKDEFY NRYIMKSFLF EPVVKAFLNN GSRYNLMNSA 600
IIEMFEFIRV EDIKSLTAHV IENYWKALED VDYVQTFKGL KLRFEQQRER QDNPKLDSMR 660
SILRNHRYRR DARTLEDEEE MWFNTDEDDM EDGEAVVSPS DKTKNDDDIM DPISKFMERK 720
KLKESEEKEV LLKTNLSGRQ SPSFKLSLSS GTKTNLTSQS STTNLPGSPG SPGSPGSPGS 780
PGSVPKNTSQ TAAITTKGGL VGLVDYPDDD EDDDEDEDKE DTLPLSKKAK FDS 833 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0030289; C:protein phosphatase 4 complex; IEA:Compara.
 GO:0045722; P:positive regulation of gluconeogenesis; IEA:Compara.
 GO:0006470; P:protein dephosphorylation; IEA:Compara. 
Interpro
 IPR016024; ARM-type_fold.
 IPR006887; DUF625.
 IPR011993; PH_like_dom. 
Pfam
 PF04802; SMK-1 
SMART
  
PROSITE
 PS50229; WH1 
PRINTS