CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022913
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor 20 
Protein Synonyms/Alias
 TCF-20; Nuclear factor SPBP; Protein AR1; Stromelysin-1 PDGF-responsive element-binding protein; SPRE-binding protein 
Gene Name
 TCF20 
Gene Synonyms/Alias
 KIAA0292; SPBP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
844TDYPIPRKFEIEPQSubiquitination[1, 2]
1086LNSQLHYKRQMYQQQubiquitination[1, 2]
1267FISPIPSKRQSQDVKubiquitination[1, 2]
1428NQELHVEKPLPRSSEacetylation[3]
1615QEPEIKLKYATQPLDubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Transcriptional activator that binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator. 
Sequence Annotation
 DNA_BIND 1537 1551 A.T hook.
 ZN_FING 1884 1935 PHD-type; atypical.
 REGION 1170 1191 Leucine-zipper.
 MOTIF 1254 1268 Nuclear localization signal (By
 MOTIF 1576 1600 Nuclear localization signal (By
 MOTIF 1785 1792 Nuclear localization signal (By
 MOD_RES 419 419 Phosphoserine.
 MOD_RES 430 430 Phosphoserine.
 MOD_RES 559 559 Phosphoserine (By similarity).
 MOD_RES 574 574 Phosphoserine.
 MOD_RES 583 583 Phosphoserine.
 MOD_RES 871 871 Phosphoserine.
 MOD_RES 966 966 Phosphoserine.
 MOD_RES 1053 1053 Phosphoserine.
 MOD_RES 1335 1335 Phosphoserine.
 MOD_RES 1361 1361 Phosphoserine.
 MOD_RES 1522 1522 Phosphoserine.
 MOD_RES 1669 1669 Phosphoserine.
 MOD_RES 1671 1671 Phosphothreonine.  
Keyword
 Activator; Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1960 AA 
Protein Sequence
MQSFREQSSY HGNQQSYPQE VHGSSRLEEF SPRQAQMFQN FGGTGGSSGS SGSGSGGGRR 60
GAAAAAAAMA SETSGHQGYQ GFRKEAGDFY YMAGNKDPVT TGTPQPPQRR PSGPVQSYGP 120
PQGSSFGNQY GSEGHVGQFQ AQHSGLGGVS HYQQDYTGPF SPGSAQYQQQ ASSQQQQQQV 180
QQLRQQLYQS HQPLPQATGQ PASSSSHLQP MQRPSTLPSS AAGYQLRVGQ FGQHYQSSAS 240
SSSSSSFPSP QRFSQSGQSY DGSYNVNAGS QYEGHNVGSN AQAYGTQSNY SYQPQSMKNF 300
EQAKIPQGTQ QGQQQQQPQQ QQHPSQHVMQ YTNAATKLPL QSQVGQYNQP EVPVRSPMQF 360
HQNFSPISNP SPAASVVQSP SCSSTPSPLM QTGENLQCGQ GSVPMGSRNR ILQLMPQLSP 420
TPSMMPSPNS HAAGFKGFGL EGVPEKRLTD PGLSSLSALS TQVANLPNTV QHMLLSDALT 480
PQKKTSKRPS SSKKADSCTN SEGSSQPEEQ LKSPMAESLD GGCSSSSEDQ GERVRQLSGQ 540
STSSDTTYKG GASEKAGSSP AQGAQNEPPR LNASPAAREE ATSPGAKDMP LSSDGNPKVN 600
EKTVGVIVSR EAMTGRVEKP GGQDKGSQED DPAATQRPPS NGGAKETSHA SLPQPEPPGG 660
GGSKGNKNGD NNSNHNGEGN GQSGHSAAGP GFTSRTEPSK SPGSLRYSYK DSFGSAVPRN 720
VSGFPQYPTG QEKGDFTGHG ERKGRNEKFP SLLQEVLQGY HHHPDRRYSR STQEHQGMAG 780
SLEGTTRPNV LVSQTNELAS RGLLNKSIGS LLENPHWGPW ERKSSSTAPE MKQINLTDYP 840
IPRKFEIEPQ SSAHEPGGSL SERRSVICDI SPLRQIVRDP GAHSLGHMSA DTRIGRNDRL 900
NPTLSQSVIL PGGLVSMETK LKSQSGQIKE EDFEQSKSQA SFNNKKSGDH CHPPSIKHES 960
YRGNASPGAA THDSLSDYGP QDSRPTPMRR VPGRVGGREG MRGRSPSQYH DFAEKLKMSP 1020
GRSRGPGGDP HHMNPHMTFS ERANRSSLHT PFSPNSETLA SAYHANTRAH AYGDPNAGLN 1080
SQLHYKRQMY QQQPEEYKDW SSGSAQGVIA AAQHRQEGPR KSPRQQQFLD RVRSPLKNDK 1140
DGMMYGPPVG TYHDPSAQEA GRCLMSSDGL PNKGMELKHG SQKLQESCWD LSRQTSPAKS 1200
SGPPGMSSQK RYGPPHETDG HGLAEATQSS KPGSVMLRLP GQEDHSSQNP LIMRRRVRSF 1260
ISPIPSKRQS QDVKNSSTED KGRLLHSSKE GADKAFNSYA HLSHSQDIKS IPKRDSSKDL 1320
PSPDSRNCPA VTLTSPAKTK ILPPRKGRGL KLEAIVQKIT SPNIRRSASS NSAEAGGDTV 1380
TLDDILSLKS GPPEGGSVAV QDADIEKRKG EVASDLVSPA NQELHVEKPL PRSSEEWRGS 1440
VDDKVKTETH AETVTAGKEP PGAMTSTTSQ KPGSNQGRPD GSLGGTAPLI FPDSKNVPPV 1500
GILAPEANPK AEEKENDTVT ISPKQEGFPP KGYFPSGKKK GRPIGSVNKQ KKQQQPPPPP 1560
PQPPQIPEGS ADGEPKPKKQ RQRRERRKPG AQPRKRKTKQ AVPIVEPQEP EIKLKYATQP 1620
LDKTDAKNKS FYPYIHVVNK CELGAVCTII NAEEEEQTKL VRGRKGQRSL TPPPSSTESK 1680
ALPASSFMLQ GPVVTESSVM GHLVCCLCGK WASYRNMGDL FGPFYPQDYA ATLPKNPPPK 1740
RATEMQSKVK VRHKSASNGS KTDTEEEEEQ QQQQKEQRSL AAHPRFKRRH RSEDCGGGPR 1800
SLSRGLPCKK AATEGSSEKT VLDSKPSVPT TSEGGPELEL QIPELPLDSN EFWVHEGCIL 1860
WANGIYLVCG RLYGLQEALE IAREMKCSHC QEAGATLGCY NKGCSFRYHY PCAIDADCLL 1920
HEENFSVRCP KHKPPLPCPL PPLQNKTAKG SLSTEQSERG 1960 
Gene Ontology
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0003713; F:transcription coactivator activity; NAS:UniProtKB.
 GO:0044212; F:transcription regulatory region DNA binding; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001965; Znf_PHD. 
Pfam
  
SMART
 SM00249; PHD 
PROSITE
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2 
PRINTS