UniProt Accession

 ERI3_HUMAN; O43414; B1AK98; Q5T2T7; Q5T2T9; Q5TG35; Q9BQA0; Q9UEB4 

Genbank Protein ID

 AF007157; AK290567; AL390776; AL035417; AL390776; AL035417; AL035417; AL390776; AL035417; AL390776; AC004254; BC001072; BC004456 

Genbank Nucleotide ID

 AAC19158.1; BAF83256.1; CAI12491.1; CAI12491.1; CAI12494.1; CAI12494.1; CAI23194.1; CAI23194.1; CAI23196.1; CAI23196.1; AAC04618.1; AAH01072.1; AAH04456.1 

Protein Name

 ERI1 exoribonuclease 3 

Protein Synonyms/Alias

 Prion interactor 1; Prion protein-interacting protein 

Gene Name

 ERI3 

Gene Synonyms/Alias

 PINT1; PRNPIP; PRNPIP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
230	RVDEWMAKEGLLDPN	ubiquitination	[1, 2]
276	FKQWINLKKAYSFAM	ubiquitination	[1, 2]
321	KNIANIMKTLAYRGF	ubiquitination	[1, 3, 4, 5]
331	AYRGFIFKQTSKPF*	ubiquitination	[2, 3, 5]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

  

Sequence Annotation

 DOMAIN 146 320 Exonuclease.
 ACT_SITE 152 152 Proton acceptor (Potential).
 ACT_SITE 307 307 Proton acceptor (Potential).
 METAL 150 150 Magnesium 1 (By similarity).
 METAL 150 150 Magnesium 2 (By similarity).
 METAL 152 152 Magnesium 1 (By similarity).
 METAL 249 249 Magnesium 2 (By similarity).
 METAL 312 312 Magnesium 1 (By similarity).
 BINDING 152 152 AMP (By similarity).
 BINDING 307 307 AMP (By similarity).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 337 AA 

Protein Sequence

Gene Ontology

 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. 

Interpro

 IPR006055; Exonuclease.
 IPR013520; Exonuclease_RNaseT/DNA_pol3.
 IPR012337; RNaseH-like_dom. 

Pfam

 PF00929; RNase_T 

SMART

 SM00479; EXOIII 

PROSITE

  

PRINTS