CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037947
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nuclear autoantigenic sperm protein 
Protein Synonyms/Alias
  
Gene Name
 NASP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
223AVLNEQVKEAEGSSAubiquitination[1]
234GSSAEYKKEIEELKEubiquitination[2]
240KKEIEELKELLPEIRacetylation[3]
240KKEIEELKELLPEIRubiquitination[1, 2, 4, 5]
249LLPEIREKIEDAKESubiquitination[1]
254REKIEDAKESQRSGNubiquitination[1, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 268 AA 
Protein Sequence
MAMESTATAA VAAELVSADK IEDVPAPSTS ADKVERMENG VLGNALEGVH VEEEEGEKTE 60
DESLVENNDN IDETEGSEED DKENDKTEEM PNDSVLENKS LQENEEEEIG NLELAWDMLD 120
LAKIIFKRQE TKEAQLYAAQ AHLKLGEVSV ESENYVQAVE EFQSCLNLQE QYLEAHDRLL 180
AETHYQLGLA YGYNSQYDEA VAQFSKSIEV IENRMAVLNE QVKEAEGSSA EYKKEIEELK 240
ELLPEIREKI EDAKESQRSG NVAELALK 268 
Gene Ontology
  
Interpro
 IPR019544; Tetratricopeptide_SHNi-TPR_dom.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF10516; SHNi-TPR
 PF00515; TPR_1 
SMART
  
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS