UniProt Accession

 ISPH_ECOLI; P62623; P22565 

Genbank Protein ID

 AY062212; X54945; U00096; AP009048 

Genbank Nucleotide ID

 AAL38655.1; CAA38707.1; AAC73140.1; BAB96598.1 

Protein Name

 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 

Protein Synonyms/Alias

  

Gene Name

 ispH 

Gene Synonyms/Alias

 lytB; yaaE; b0029; JW0027 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
249	AFLIDDAKDIQEEWV	acetylation	[1]
257	DIQEEWVKEVKCVGV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth. 

Sequence Annotation

 REGION 225 227 Substrate binding.
 METAL 12 12 Iron-sulfur (3Fe-4S).
 METAL 96 96 Iron-sulfur (3Fe-4S).
 METAL 197 197 Iron-sulfur (3Fe-4S).
 BINDING 41 41 Substrate.
 BINDING 74 74 Substrate.
 BINDING 124 124 Substrate.
 BINDING 167 167 Substrate.
 BINDING 269 269 Substrate.  

Keyword

 3D-structure; 3Fe-4S; Complete proteome; Iron; Iron-sulfur; Isoprene biosynthesis; Metal-binding; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 316 AA 

Protein Sequence

Gene Ontology

 GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:HAMAP.
 GO:0051745; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity; IEA:HAMAP.
 GO:0042380; F:hydroxymethylbutenyl pyrophosphate reductase activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050992; P:dimethylallyl diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0019288; P:isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; IEA:HAMAP.
 GO:0046677; P:response to antibiotic; IDA:EcoCyc.
 GO:0016114; P:terpenoid biosynthetic process; IEA:HAMAP.
 GO:0006744; P:ubiquinone biosynthetic process; IDA:EcoCyc. 

Interpro

 IPR003451; LytB. 

Pfam

 PF02401; LYTB 

SMART

  

PROSITE

  

PRINTS