CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004004
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histidine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Histidyl-tRNA synthetase; HisRS 
Gene Name
 HARS 
Gene Synonyms/Alias
 HRS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12AALEELVKLQGERVRubiquitination[1]
42VAKLLKLKAQLGPDEubiquitination[1, 2]
51QLGPDESKQKFVLKTubiquitination[2]
53GPDESKQKFVLKTPKubiquitination[1]
57SKQKFVLKTPKGTRDubiquitination[1]
75RQMAVREKVFDVIIRubiquitination[1, 3, 4]
106LKETLMGKYGEDSKLubiquitination[1, 2]
112GKYGEDSKLIYDLKDubiquitination[1, 2]
118SKLIYDLKDQGGELLubiquitination[1, 2, 5]
143ARYLAMNKLTNIKRYubiquitination[1]
230ICGVSDSKFRTICSSubiquitination[1, 5]
250KVSWEEVKNEMVGEKubiquitination[1, 2, 5]
257KNEMVGEKGLAPEVAubiquitination[1, 2, 5]
288EQLLQDPKLSQNKQAubiquitination[1, 2]
293DPKLSQNKQALEGLGubiquitination[2, 3, 4, 5]
373LVGMFDPKGRKVPCVubiquitination[1, 2]
376MFDPKGRKVPCVGLSubiquitination[1]
403RLEALEEKIRTTETQubiquitination[1, 2]
418VLVASAQKKLLEERLubiquitination[1, 2]
426KLLEERLKLVSELWDubiquitination[1]
443IKAELLYKKNPKLLNacetylation[6]
477ELKDGVIKLRSVTSRubiquitination[2]
499EDLVEEIKRRTGQPLubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
  
Sequence Annotation
 DOMAIN 3 59 WHEP-TRS.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Deafness; Disease mutation; Ligase; Neuropathy; Nucleotide-binding; Polymorphism; Protein biosynthesis; Reference proteome; Retinitis pigmentosa; Usher syndrome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 509 AA 
Protein Sequence
MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK 60
GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK ETLMGKYGED SKLIYDLKDQ 120
GGELLSLRYD LTVPFARYLA MNKLTNIKRY HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG 180
NFDPMIPDAE CLKIMCEILS SLQIGDFLVK VNDRRILDGM FAICGVSDSK FRTICSSVDK 240
LDKVSWEEVK NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG 300
DLKLLFEYLT LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQTPAQAGEE PLGVGSVAAG 360
GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL EEKIRTTETQ VLVASAQKKL 420
LEERLKLVSE LWDAGIKAEL LYKKNPKLLN QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS 480
VTSREEVDVR REDLVEEIKR RTGQPLCIC 509 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0004821; F:histidine-tRNA ligase activity; NAS:UniProtKB.
 GO:0006427; P:histidyl-tRNA aminoacylation; IEA:InterPro.
 GO:0006418; P:tRNA aminoacylation for protein translation; TAS:Reactome. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR015807; His-tRNA-ligase.
 IPR004516; HisRS/HisZ.
 IPR009068; S15_NS1_RNA-bd.
 IPR000738; WHEP-TRS. 
Pfam
 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b
 PF00458; WHEP-TRS 
SMART
 SM00991; WHEP-TRS 
PROSITE
 PS50862; AA_TRNA_LIGASE_II
 PS00762; WHEP_TRS_1
 PS51185; WHEP_TRS_2 
PRINTS