CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006743
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Malate synthase G 
Protein Synonyms/Alias
 MSG 
Gene Name
 glcB 
Gene Synonyms/Alias
 glc; b2976; JW2943 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
82VKDKAAYKSFLRELGacetylation[1]
196VAFKVVDKQLRIQLKacetylation[1]
301MQGTLQEKMEKNGRQacetylation[1, 2]
404QEVAFANKLFTRIETacetylation[1]
483MKSTPWIKAYERNNVacetylation[1]
653LRHGILTKEQVQASLacetylation[1]
665ASLENMAKVVDQQNAacetylation[1]
702DLIFLGVKQPNGYTEacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 Accounts for almost the entire malate-synthesizing activity in cells metabolizing glyoxylate. 
Sequence Annotation
 ACT_SITE 338 338 Proton acceptor.
 ACT_SITE 631 631 Proton donor.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Glyoxylate bypass; Magnesium; Reference proteome; Transferase; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 723 AA 
Protein Sequence
MSQTITQSRL RIDANFKRFV DEEVLPGTGL DAAAFWRNFD EIVHDLAPEN RQLLAERDRI 60
QAALDEWHRS NPGPVKDKAA YKSFLRELGY LVPQPERVTV ETTGIDSEIT SQAGPQLVVP 120
AMNARYALNA ANARWGSLYD ALYGSDIIPQ EGAMVSGYDP QRGEQVIAWV RRFLDESLPL 180
ENGSYQDVVA FKVVDKQLRI QLKNGKETTL RTPAQFVGYR GDAAAPTCIL LKNNGLHIEL 240
QIDANGRIGK DDPAHINDVI VEAAISTILD CEDSVAAVDA EDKILLYRNL LGLMQGTLQE 300
KMEKNGRQIV RKLNDDRHYT AADGSEISLH GRSLLFIRNV GHLMTIPVIW DSEGNEIPEG 360
ILDGVMTGAI ALYDLKVQKN SRTGSVYIVK PKMHGPQEVA FANKLFTRIE TMLGMAPNTL 420
KMGIMDEERR TSLNLRSCIA QARNRVAFIN TGFLDRTGDE MHSVMEAGPM LRKNQMKSTP 480
WIKAYERNNV LSGLFCGLRG KAQIGKGMWA MPDLMADMYS QKGDQLRAGA NTAWVPSPTA 540
ATLHALHYHQ TNVQSVQANI AQTEFNAEFE PLLDDLLTIP VAENANWSAQ EIQQELDNNV 600
QGILGYVVRW VEQGIGCSKV PDIHNVALME DRATLRISSQ HIANWLRHGI LTKEQVQASL 660
ENMAKVVDQQ NAGDPAYRPM AGNFANSCAF KAASDLIFLG VKQPNGYTEP LLHAWRLREK 720
ESH 723 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004474; F:malate synthase activity; IDA:EcoCyc.
 GO:0009436; P:glyoxylate catabolic process; IEP:EcoCyc.
 GO:0006097; P:glyoxylate cycle; IEA:HAMAP.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 
Interpro
 IPR011076; Malate_synth-like.
 IPR023310; Malate_synth_G_beta_sub_dom.
 IPR001465; Malate_synthase.
 IPR006253; Malate_synthG. 
Pfam
 PF01274; Malate_synthase 
SMART
  
PROSITE
  
PRINTS