UniProt Accession

 RMLB2_ECOLI; P27830; P76754; Q2M896; Q6BF01 

Genbank Protein ID

 M87049; U00096; AP009048 

Genbank Nucleotide ID

 AAA67588.1; AAT48213.1; BAE77510.1 

Protein Name

 dTDP-glucose 4,6-dehydratase 2 

Protein Synonyms/Alias

  

Gene Name

 rffG 

Gene Synonyms/Alias

 b3788; JW5598 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
123	WNALTEDKKSAFRFH	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction. 

Sequence Annotation

 NP_BIND 8 14 NAD.
 NP_BIND 33 36 NAD.
 NP_BIND 59 60 NAD.
 NP_BIND 160 164 NAD.
 REGION 134 136 Substrate binding (By similarity).
 REGION 199 200 Substrate binding (By similarity).
 REGION 215 217 Substrate binding (By similarity).
 REGION 294 297 Substrate binding (By similarity).
 ACT_SITE 135 135 Proton donor.
 ACT_SITE 136 136 Proton acceptor.
 ACT_SITE 160 160 Proton acceptor.
 BINDING 81 81 NAD; via carbonyl oxygen.
 BINDING 85 85 Substrate; via carbonyl oxygen (By
 BINDING 100 100 NAD.
 BINDING 189 189 Substrate (By similarity).
 BINDING 190 190 NAD; via amide nitrogen.
 BINDING 224 224 Substrate (By similarity).
 BINDING 259 259 Substrate (By similarity).  

Keyword

 3D-structure; Complete proteome; Lipopolysaccharide biosynthesis; Lyase; NAD; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 355 AA 

Protein Sequence

Gene Ontology

 GO:0050662; F:coenzyme binding; IEA:InterPro.
 GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IDA:UniProtKB.
 GO:0019305; P:dTDP-rhamnose biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0045226; P:extracellular polysaccharide biosynthetic process; IDA:UniProtKB.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IDA:UniProtKB.
 GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR005888; dTDP_Gluc_deHydtase.
 IPR001509; Epimerase_deHydtase.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF01370; Epimerase 

SMART

  

PROSITE

  

PRINTS