UniProt Accession

 CNOT7_HUMAN; Q9UIV1; A8MZM5; B3KMP1; B3KN35; D3DSP6; G3V108; Q7Z530 

Genbank Protein ID

 L46722; AF086915; AK021808; AK023466; AC091050; CH471080; CH471080; CH471080; CH471080; BC060852; BC070187 

Genbank Nucleotide ID

 AAF01500.1; AAP97145.1; BAG51053.1; BAG51197.1; EAW63820.1; EAW63821.1; EAW63822.1; EAW63823.1; AAH60852.1; AAH70187.1 

Protein Name

 CCR4-NOT transcription complex subunit 7 

Protein Synonyms/Alias

 BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a 

Gene Name

 CNOT7 

Gene Synonyms/Alias

 CAF1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	KIRQVIRKYNYVAMD	ubiquitination	[1]
206	MKSCKNLKGGLQEVA	ubiquitination	[1, 2, 3]
240	LTGMAFFKMREMFFE	ubiquitination	[1, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT8. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti- proliferative activity. 

Sequence Annotation

 METAL 40 40 Divalent metal cation 1; catalytic
 METAL 40 40 Divalent metal cation 2; catalytic
 METAL 42 42 Divalent metal cation 2; catalytic
 METAL 161 161 Divalent metal cation 1; catalytic
 METAL 230 230 Divalent metal cation 2; catalytic
 METAL 278 278 Divalent metal cation 1; catalytic  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Reference proteome; Repressor; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 285 AA 

Protein Sequence

Gene Ontology

 GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
 GO:0000932; C:cytoplasmic mRNA processing body; ISS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004535; F:poly(A)-specific ribonuclease activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:ProtInc.
 GO:0033962; P:cytoplasmic mRNA processing body assembly; IEA:Compara.
 GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IEA:Compara.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; IDA:UniProtKB.
 GO:0035195; P:gene silencing by miRNA; TAS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB.
 GO:1900153; P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IMP:UniProtKB.
 GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR006941; RNase_CAF1.
 IPR012337; RNaseH-like_dom. 

Pfam

 PF04857; CAF1 

SMART

  

PROSITE

  

PRINTS