CPLM-001039

Genbank Nucleotide ID

Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma

Protein Synonyms/Alias

PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; Phosphatidylinositol 4-phosphate 5-kinase type I gamma; PIP5KIgamma

Mus musculus (Mouse)

Position	Peptide	Type	References
265	TYKRRASKKEKEKSL	acetylation	[1]
268	RRASKKEKEKSLPTY	acetylation	[1]

[1] SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5Kgamma Activity through Deacetylation of Specific Lysine Residues in Mammals.
Akieda-Asai S, Zaima N, Ikegami K, Kahyo T, Yao I, Hatanaka T, Iemura S, Sugiyama R, Yokozeki T, Eishi Y, Koike M, Ikeda K, Chiba T, Yamaza H, Shimokawa I, Song SY, Matsuno A, Mizutani A, Sawabe M, Chao MV, Tanaka M, Kanaho Y, Natsume T, Sugimura H, Date Y, McBurney MW, Guarente L, Setou M.
PLoS One. 2010 Jul 23;5(7):e11755. [PMID: 20668706]

Functional Description

Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A have a role during embryogenesis and together with PIP5K1B may have a role immediately after birth.

DOMAIN 75 443 PIPK.
REGION 636 661 Mediates interaction with TLN2.
MOD_RES 265 265 N6-acetyllysine (By similarity).
MOD_RES 268 268 N6-acetyllysine (By similarity).
MOD_RES 634 634 Phosphotyrosine; by EGFR.
MOD_RES 644 644 Phosphotyrosine; by CSK.
MOD_RES 645 645 Phosphoserine; by CDK5, MAPK1 and CDK1

3D-structure; Acetylation; Alternative splicing; ATP-binding; Cell adhesion; Cell junction; Cell membrane; Cell projection; Chemotaxis; Complete proteome; Cytoplasm; Endocytosis; Exocytosis; Kinase; Membrane; Nucleotide-binding; Phagocytosis; Phosphoprotein; Reference proteome; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
GO:0001891; C:phagocytic cup; IEA:UniProtKB-SubCell.
GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
GO:0001931; C:uropod; IEA:UniProtKB-SubCell.
GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IDA:MGI.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0007409; P:axonogenesis; IDA:MGI.
GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
GO:0006909; P:phagocytosis; IEA:UniProtKB-KW.

IPR023610; PInositol-4-P-5-kinase.
IPR027483; PInositol-4-P-5-kinase_C.
IPR002498; PInositol-4-P-5-kinase_core.
IPR027484; PInositol-4-P-5-kinase_N.
IPR016034; PInositol-4P-5-kinase_core_sub.