CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000670
UniProt Accession
Genbank Protein ID
 U54996 
Genbank Nucleotide ID
Protein Name
 Centromere/kinetochore protein zw10 homolog 
Protein Synonyms/Alias
  
Gene Name
 ZW10 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
17AHSGRLEKEDLGTRIubiquitination[1]
74SEDIDLLKSRIESEVubiquitination[1, 2]
96TGEFTDLKQQLERDSubiquitination[1, 2]
130NCALTEKKYVTGAQRubiquitination[1]
143QRLEEAQKCLKLLKSubiquitination[1]
382LKEMRFLKGDTTDLLubiquitination[1]
390GDTTDLLKYARNINSubiquitination[1]
403NSHFANKKCQDVIVAubiquitination[1]
484CRISESVKKLMELAYubiquitination[2]
597LAQMRAQKGELLERLubiquitination[1]
624ENYSAASKAVRQVLHubiquitination[1, 2]
777RRAAALAKIK*****acetylation[3, 4]
777RRAAALAKIK*****ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 
Functional Description
 Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum. 
Sequence Annotation
 REGION 2 317 Interaction with RINT1.
 REGION 2 81 Interaction with ZWINT.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 777 777 N6-acetyllysine.  
Keyword
 Acetylation; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Endoplasmic reticulum; ER-Golgi transport; Kinetochore; Membrane; Mitosis; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 779 AA 
Protein Sequence
MASFVTEVLA HSGRLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL PSMQSAQGLI 60
TQVDKLSEDI DLLKSRIESE VRRDLHVSTG EFTDLKQQLE RDSVVLSLLK QLQEFSTAIE 120
EYNCALTEKK YVTGAQRLEE AQKCLKLLKS RKCFDLKILK SLSMELTIQK QNILYHLGEE 180
WQKLIVWKFP PSKDTSSLES YLQTELHLYT EQSHKEEKTP MPPISSVLLA FSVLGELHSK 240
LKSFGQMLLK YILRPLASCP SLHAVIESQP NIVIIRFESI MTNLEYPSPS EVFTKIRLVL 300
EVLQKQLLDL PLDTDLENEK TSTVPLAEML GDMIWEDLSE CLIKNCLVYS IPTNSSKLQQ 360
YEEIIQSTEE FENALKEMRF LKGDTTDLLK YARNINSHFA NKKCQDVIVA ARNLMTSEIH 420
NTVKIIPDSK INVPELPTPD EDNKLEVQKV SNTQYHEVMN LEPENTLDQH SFSLPTCRIS 480
ESVKKLMELA YQTLLEATTS SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LQKLPQLAAI 540
HHNNCMYIAH HLLTLGHQFR LRLAPILCDG TATFVDLVPG FRRLGTECFL AQMRAQKGEL 600
LERLSSARNF SNMDDEENYS AASKAVRQVL HQLKRLGIVW QDVLPVNIYC KAMGTLLNTA 660
ISEVIGKITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL SEESKNKKYQ EEVPVYVPKW 720
MPFKELMMML QASLQEIGDR WADGKGPLAA AFSSSEVKAL IRALFQNTER RAAALAKIK 779 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005783; C:endoplasmic reticulum; IDA:HGNC.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0005828; C:kinetochore microtubule; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0019237; F:centromeric DNA binding; TAS:ProtInc.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:HGNC.
 GO:0000132; P:establishment of mitotic spindle orientation; IMP:UniProtKB.
 GO:0007126; P:meiosis; TAS:ProtInc.
 GO:0007093; P:mitotic cell cycle checkpoint; IDA:UniProtKB.
 GO:0007080; P:mitotic metaphase plate congression; IMP:UniProtKB.
 GO:0006461; P:protein complex assembly; IDA:HGNC.
 GO:0034501; P:protein localization to kinetochore; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0007096; P:regulation of exit from mitosis; IDA:HGNC. 
Interpro
 IPR009361; RZZ-complex_Zw10. 
Pfam
 PF06248; Zw10 
SMART
  
PROSITE
  
PRINTS