UniProt Accession

 RSGA_ECOLI; P39286; Q2M6E1 

Genbank Protein ID

 U14003; U00096; AP009048 

Genbank Nucleotide ID

 AAA97060.1; AAC77121.2; BAE78165.1 

Protein Name

 Putative ribosome biogenesis GTPase RsgA 

Protein Synonyms/Alias

  

Gene Name

 rsgA 

Gene Synonyms/Alias

 engC; yjeQ; b4161; JW4122 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
28	RLKTSKEKPDYDDNL	acetylation	[1]
85	RVVWRPGKPAAEGVN	acetylation	[1]
94	AAEGVNVKGIVEAVH	acetylation	[1]
199	SHTQDGLKPLEEALT	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. Dispensible for viability, but important for overall fitness. The intrinsic GTPase activity is stimulated by the presence of 30S (160-fold increase in kcat) or 70S (96 fold increase in kcat) ribosomes (PubMed:14973029). The GTPase is inhibited by aminoglycoside antibiotics such as neomycin and paromycin (PubMed:15466596) streptomycin and spectinomycin (PubMed:15828870). This inhibition is not due to competition for binding sites on the 30S or 70S ribosome (PubMed:15828870). 

Sequence Annotation

 DOMAIN 121 271 EngC GTPase.
 NP_BIND 160 163 GTP (By similarity).
 NP_BIND 214 222 GTP (By similarity).
 REGION 1 113 Necessary for association with the
 REGION 1 20 Necessary for GMP-PNP-dependent
 MOTIF 297 310 Knuckle-like cysteine cluster.
 METAL 297 297 Zinc (By similarity).
 METAL 302 302 Zinc (By similarity).
 METAL 304 304 Zinc (By similarity).
 METAL 310 310 Zinc (By similarity).  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; GTP-binding; Hydrolase; Metal-binding; Nucleotide-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 350 AA 

Protein Sequence

Gene Ontology

 GO:0019003; F:GDP binding; IDA:EcoCyc.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0003924; F:GTPase activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042274; P:ribosomal small subunit biogenesis; IGI:EcoCyc. 

Interpro

 IPR010914; EngC_GTPase.
 IPR027417; P-loop_NTPase.
 IPR004881; Ribosome_biogen_GTPase_RsgA. 

Pfam

 PF03193; DUF258 

SMART

  

PROSITE

 PS50936; ENGC_GTPASE 

PRINTS