UniProt Accession

 VP33B_HUMAN; Q9H267; Q96K14; Q9NRP6; Q9NSF3 

Genbank Protein ID

 AF201694; AL357472; AF308803; AK027754; AC068831; BC016445 

Genbank Nucleotide ID

 AAF91174.1; CAB93109.1; AAG34680.1; BAB55345.1; AAH16445.1 

Protein Name

 Vacuolar protein sorting-associated protein 33B 

Protein Synonyms/Alias

 hVPS33B 

Gene Name

 VPS33B 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	LPDFSMLKRLARDQL	ubiquitination	[1]
36	LLEQLPGKKDLFIEA	ubiquitination	[2]
201	YGIGRCAKMAYELWR	ubiquitination	[1]
219	EEEDGETKGRRPEIG	ubiquitination	[2]
258	VDDTFRIKCGSVDFG	ubiquitination	[1]
304	VFGFLSQKARNLQAQ	ubiquitination	[1, 3]
458	TLTAVESKVSKLVTD	ubiquitination	[1, 2]
461	AVESKVSKLVTDKAA	ubiquitination	[1]
466	VSKLVTDKAAGKITD	ubiquitination	[1]
480	DAFSSLAKRSNFRAI	ubiquitination	[1, 2]
504	VDGEYDLKVPRDMAY	ubiquitination	[1, 2]
616	MEAMSEVKA******	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Complete proteome; Disease mutation; Endosome; Lysosome; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 617 AA 

Protein Sequence

Gene Ontology

 GO:0005770; C:late endosome; IDA:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0031091; C:platelet alpha granule; IDA:UniProtKB.
 GO:0006944; P:cellular membrane fusion; IMP:UniProtKB.
 GO:0032418; P:lysosome localization; IDA:UniProtKB.
 GO:0032400; P:melanosome localization; IDA:UniProtKB.
 GO:0070889; P:platelet alpha granule organization; IMP:UniProtKB.
 GO:0015031; P:protein transport; IMP:UniProtKB.
 GO:0006904; P:vesicle docking involved in exocytosis; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IDA:MGI. 

Interpro

 IPR027482; Sec-1-like_dom2.
 IPR001619; Sec1-like.
 IPR027121; VPS33. 

Pfam

 PF00995; Sec1 

SMART

  

PROSITE

  

PRINTS