CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002641
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phenylalanine--tRNA ligase alpha subunit 
Protein Synonyms/Alias
 Phenylalanyl-tRNA synthetase alpha subunit; PheRS 
Gene Name
 pheS 
Gene Synonyms/Alias
 b1714; JW5277 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
34VRVEYLGKKGHLTLQacetylation[1]
64GAVINEAKEQVQQALacetylation[1]
75QQALNARKAELESAAacetylation[1]
179GVQIRTMKAQQPPIRacetylation[1]
262AEVDVMGKNGKWLEVacetylation[1]
265DVMGKNGKWLEVLGCacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 METAL 252 252 Magnesium (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 327 AA 
Protein Sequence
MSHLAELVAS AKAAISQASD VAALDNVRVE YLGKKGHLTL QMTTLRELPP EERPAAGAVI 60
NEAKEQVQQA LNARKAELES AALNARLAAE TIDVSLPGRR IENGGLHPVT RTIDRIESFF 120
GELGFTVATG PEIEDDYHNF DALNIPGHHP ARADHDTFWF DTTRLLRTQT SGVQIRTMKA 180
QQPPIRIIAP GRVYRNDYDQ THTPMFHQME GLIVDTNISF TNLKGTLHDF LRNFFEEDLQ 240
IRFRPSYFPF TEPSAEVDVM GKNGKWLEVL GCGMVHPNVL RNVGIDPEVY SGFAFGMGME 300
RLTMLRYGVT DLRSFFENDL RFLKQFK 327 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004826; F:phenylalanine-tRNA ligase activity; IEA:HAMAP.
 GO:0000049; F:tRNA binding; IEA:InterPro.
 GO:0006432; P:phenylalanyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR006195; aa-tRNA-synth_II.
 IPR004529; Phe-tRNA-synth_IIc_asu.
 IPR004188; Phe-tRNA_ligase_II_N.
 IPR022911; Phe_tRNA_ligase_alpha1_bac.
 IPR002319; Phenylalanyl-tRNA_Synthase.
 IPR010978; tRNA-bd_arm. 
Pfam
 PF02912; Phe_tRNA-synt_N
 PF01409; tRNA-synt_2d 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS