CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004498
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phosphofructokinase subunit alpha 
Protein Synonyms/Alias
 6PF-1-K subunit alpha; Phosphofructokinase 1; Phosphohexokinase 
Gene Name
 PFK1 
Gene Synonyms/Alias
 YGR240C; G8599 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
24TNDEALFKKTIHFYHacetylation[1]
45VKDFNKFKHGENSLLacetylation[1]
89PQQEATNKAQSQGALacetylation[1]
89PQQEATNKAQSQGALubiquitination[2, 3]
132DLNKIVEKFPKQAEKacetylation[1]
139KFPKQAEKLSDTLVFacetylation[1]
207GSSQKKKKIAVMTSGubiquitination[3]
337VAEGRFTKEEVAPYKacetylation[1]
337VAEGRFTKEEVAPYKubiquitination[3]
387DYIDATAKSHSRAFVubiquitination[3]
429ERAVPHGKWQDELKEacetylation[1]
532LIGILENKIIRMPLVubiquitination[3]
546VESVKLTKSVATAIEacetylation[1]
555VATAIENKDFDKAISacetylation[1]
559IENKDFDKAISLRDTacetylation[1]
559IENKDFDKAISLRDTubiquitination[2, 3]
625YCLSHGHKPYAIMNGubiquitination[2]
807REDITLLKENFRHDKacetylation[1]
821KGENRNGKLLVRNEQubiquitination[3]
890KFIEQWNKKNEASPNacetylation[1]
901ASPNTDAKVLRFKFDacetylation[1]
901ASPNTDAKVLRFKFDubiquitination[3]
906DAKVLRFKFDTHGEKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 NP_BIND 225 229 ATP (By similarity).
 NP_BIND 383 387 ATP (By similarity).
 NP_BIND 400 416 ATP (By similarity).
 ACT_SITE 356 356 Proton acceptor (By similarity).
 METAL 414 414 Magnesium; via carbonyl oxygen (By
 BINDING 391 391 Substrate (By similarity).
 BINDING 488 488 Substrate (By similarity).
 BINDING 491 491 Substrate (By similarity).
 MOD_RES 3 3 Phosphoserine.
 MOD_RES 166 166 Phosphoserine.
 MOD_RES 179 179 Phosphoserine.
 MOD_RES 185 185 Phosphoserine.
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 192 192 Phosphoserine.
 MOD_RES 217 217 Phosphoserine.
 MOD_RES 450 450 Phosphothreonine.  
Keyword
 3D-structure; Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 987 AA 
Protein Sequence
MQSQDSCYGV AFRSIITNDE ALFKKTIHFY HTLGFATVKD FNKFKHGENS LLSSGTSQDS 60
LREVWLESFK LSEVDASGFR IPQQEATNKA QSQGALLKIR LVMSAPIDET FDTNETATIT 120
YFSTDLNKIV EKFPKQAEKL SDTLVFLKDP MGNNITFSGL ANATDSAPTS KDAFLEATSE 180
DEIISRASSD ASDLLRQTLG SSQKKKKIAV MTSGGDSPGM NAAVRAVVRT GIHFGCDVFA 240
VYEGYEGLLR GGKYLKKMAW EDVRGWLSEG GTLIGTARSM EFRKREGRRQ AAGNLISQGI 300
DALVVCGGDG SLTGADLFRH EWPSLVDELV AEGRFTKEEV APYKNLSIVG LVGSIDNDMS 360
GTDSTIGAYS ALERICEMVD YIDATAKSHS RAFVVEVMGR HCGWLALMAG IATGADYIFI 420
PERAVPHGKW QDELKEVCQR HRSKGRRNNT IIVAEGALDD QLNPVTANDV KDALIELGLD 480
TKVTILGHVQ RGGTAVAHDR WLATLQGVDA VKAVLEFTPE TPSPLIGILE NKIIRMPLVE 540
SVKLTKSVAT AIENKDFDKA ISLRDTEFIE LYENFLSTTV KDDGSELLPV SDRLNIGIVH 600
VGAPSAALNA ATRAATLYCL SHGHKPYAIM NGFSGLIQTG EVKELSWIDV ENWHNLGGSE 660
IGTNRSVASE DLGTIAYYFQ KNKLDGLIIL GGFEGFRSLK QLRDGRTQHP IFNIPMCLIP 720
ATVSNNVPGT EYSLGVDTCL NALVNYTDDI KQSASATRRR VFVCEVQGGH SGYIASFTGL 780
ITGAVSVYTP EKKIDLASIR EDITLLKENF RHDKGENRNG KLLVRNEQAS SVYSTQLLAD 840
IISEASKGKF GVRTAIPGHV QQGGVPSSKD RVTASRFAVK CIKFIEQWNK KNEASPNTDA 900
KVLRFKFDTH GEKVPTVEHE DDSAAVICVN GSHVSFKPIA NLWENETNVE LRKGFEVHWA 960
EYNKIGDILS GRLKLRAEVA ALAAENK 987 
Gene Ontology
 GO:0005945; C:6-phosphofructokinase complex; IMP:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0003872; F:6-phosphofructokinase activity; IMP:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006002; P:fructose 6-phosphate metabolic process; IEA:InterPro.
 GO:0006096; P:glycolysis; IDA:SGD.
 GO:0015992; P:proton transport; IGI:UniProtKB. 
Interpro
 IPR009161; 6-phosphofructokinase_euk.
 IPR022953; Phosphofructokinase.
 IPR015912; Phosphofructokinase_CS.
 IPR000023; Phosphofructokinase_dom. 
Pfam
 PF00365; PFK 
SMART
  
PROSITE
 PS00433; PHOSPHOFRUCTOKINASE 
PRINTS
 PR00476; PHFRCTKINASE.