CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002780
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 C-1-tetrahydrofolate synthase, mitochondrial 
Protein Synonyms/Alias
 C1-THF synthase; Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase; Formyltetrahydrofolate synthetase 
Gene Name
 MIS1 
Gene Synonyms/Alias
 YBR084W; YBR0751 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
114ITEVELLKKISDINDacetylation[1]
191LLEEAHVKLDGKNAVacetylation[1]
291DVDFDSVKEKTSYITacetylation[1]
329RQFVESEKLPVIKPLacetylation[1]
431ALSAHLGKPSIANVRubiquitination[2]
506KNDSTFYKRLVPRKKacetylation[1]
582IGEAATEKGFTRTTGacetylation[1]
895KKIDTYKKQGFGNLPacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 NP_BIND 201 203 NADP (By similarity).
 NP_BIND 408 415 ATP (By similarity).
 REGION 35 343 Methylenetetrahydrofolate dehydrogenase
 REGION 83 87 Substrate binding (By similarity).
 REGION 130 132 Substrate binding (By similarity).
 REGION 301 305 Substrate binding (By similarity).
 REGION 344 975 Formyltetrahydrofolate synthetase.
 BINDING 226 226 NADP (By similarity).  
Keyword
 Amino-acid biosynthesis; ATP-binding; Complete proteome; Direct protein sequencing; Histidine biosynthesis; Hydrolase; Ligase; Methionine biosynthesis; Mitochondrion; Multifunctional enzyme; NADP; Nucleotide-binding; One-carbon metabolism; Oxidoreductase; Purine biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 975 AA 
Protein Sequence
MLSRLSLLSN SRAFQQARWR IYRLKVSPTV HASQYHILSG RKLAQSIREK ANDEIQAIKL 60
KHPNFKPTLK IIQVGARPDS STYVRMKLKA SKDSNVDCII EKLPAEITEV ELLKKISDIN 120
DDDSIHGLLI QLPLPRHLDE TTITNAVDFK KDVDGFHRYN AGELAKKGGK PYFIPCTPYG 180
CMKLLEEAHV KLDGKNAVVL GRSSIVGNPI ASLLKNANAT VTVCHSHTRN IAEVVSQADI 240
VIAACGIPQY VKSDWIKEGA VVIDVGINYV PDISKKSGQK LVGDVDFDSV KEKTSYITPV 300
PGGVGPMTVA MLVSNVLLAA KRQFVESEKL PVIKPLPLHL ESPVPSDIDI SRAQSPKHIK 360
QVAEELGIHS HELELYGHYK AKISPNIFKR LESRENGKYV LVAGITPTPL GEGKSTTTMG 420
LVQALSAHLG KPSIANVRQP SLGPTLGVKG GAAGGGYAQV IPMDEFNLHL TGDIHAISAA 480
NNLLAAAIDT RMFHEATQKN DSTFYKRLVP RKKGIRKFTP SMQRRLKRLD IEKEDPDALT 540
PEEVKRFARL NINPDTITIR RVVDINDRML RQITIGEAAT EKGFTRTTGF DITVASELMA 600
ILALSKSLHE MKERIGRMVI GADYDNKPVT VEDIGCTGAL TALLRDAIKP NLMQTLEGTP 660
VMVHAGPFAN ISIGASSVIA DLMALKLVGS EKNPLNDKNI HEPGYVVTEA GFDFAMGGER 720
FFDIKCRSSG LVPDAVVLVA TVRALKSHGG APNVKPGQSL PKEYTEENID FVAKGVSNLV 780
KQIENIKTFG IPVVVAINRF ETDSQAEIEV IKKAALNAGA SHAVTSNHWM EGGKGAVELA 840
HAVVDATKEP KNFNFLYDVN SSIEDKLTSI VQKMYGGAKI EVSPEAQKKI DTYKKQGFGN 900
LPICIAKTQY SLSHDPSLKG VPRGFTFPIR DVRASIGAGY LYALAAEIQT IPGLSTYAGY 960
MAVEVDDDGE IEGLF 975 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004329; F:formate-tetrahydrofolate ligase activity; IDA:SGD.
 GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IDA:SGD.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IDA:SGD.
 GO:0046656; P:folic acid biosynthetic process; IDA:SGD.
 GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
 GO:0006139; P:nucleobase-containing compound metabolic process; IMP:SGD.
 GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 
Interpro
 IPR000559; Formate_THF_ligase.
 IPR020628; Formate_THF_ligase_CS.
 IPR016040; NAD(P)-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020867; THF_DH/CycHdrlase_CS.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 
Pfam
 PF01268; FTHFS
 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 
SMART
  
PROSITE
 PS00721; FTHFS_1
 PS00722; FTHFS_2
 PS00766; THF_DHG_CYH_1
 PS00767; THF_DHG_CYH_2 
PRINTS
 PR00085; THFDHDRGNASE.