CPLM-004092

Genbank Nucleotide ID

Protein Synonyms/Alias

NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

Rattus norvegicus (Rat)

Position	Peptide	Type	References
27	GCELKADKDYHFKVD	acetylation	[1]
32	ADKDYHFKVDNDENE	acetylation	[1]
54	VSLGAGAKDELHIVE	acetylation	[1]
150	SAPGGGNKVPQKKVK	acetylation	[1]
154	GGNKVPQKKVKLDED	acetylation	[1]

[1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]

Functional Description

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules (By similarity). Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication (By similarity). Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation (By similarity).

REGION 1 185 Required for interaction with SENP3 (By
REGION 1 117 Necessary for interaction with APEX1 (By
REGION 241 292 Required for nucleolar localization (By
MOTIF 152 157 Nuclear localization signal (Potential).
MOTIF 190 196 Nuclear localization signal (Potential).
MOD_RES 1 1 N-acetylmethionine (By similarity).
MOD_RES 4 4 Phosphoserine; by PLK1 and PLK2 (By
MOD_RES 10 10 Phosphoserine (By similarity).
MOD_RES 32 32 N6-acetyllysine (By similarity).
MOD_RES 70 70 Phosphoserine (By similarity).
MOD_RES 75 75 Phosphothreonine (By similarity).
MOD_RES 95 95 Phosphothreonine (By similarity).
MOD_RES 125 125 Phosphoserine; by CDK2.
MOD_RES 139 139 Phosphoserine (By similarity).
MOD_RES 150 150 N6-acetyllysine (By similarity).
MOD_RES 154 154 N6-acetyllysine (By similarity).
MOD_RES 198 198 Phosphothreonine; by CDK1 and CDK2 (By
MOD_RES 211 211 N6-acetyllysine (By similarity).
MOD_RES 217 217 Phosphothreonine; by CDK1 (By
MOD_RES 225 225 Phosphoserine (By similarity).
MOD_RES 227 227 N6-acetyllysine (By similarity).
MOD_RES 228 228 N6-acetyllysine; alternate (By
MOD_RES 232 232 Phosphothreonine; by CDK1 (By
MOD_RES 235 235 Phosphothreonine; by CDK1 (By
MOD_RES 240 240 Phosphoserine (By similarity).
MOD_RES 241 241 Phosphoserine (By similarity).
MOD_RES 248 248 N6-acetyllysine (By similarity).
MOD_RES 252 252 Phosphoserine (By similarity).
MOD_RES 255 255 N6-acetyllysine (By similarity).
MOD_RES 258 258 Phosphoserine (By similarity).
MOD_RES 265 265 N6-acetyllysine (By similarity).
MOD_RES 271 271 N6-acetyllysine (By similarity).
MOD_RES 277 277 Phosphothreonine (By similarity).
MOD_RES 290 290 N6-acetyllysine (By similarity).
CROSSLNK 228 228 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 261 261 Glycyl lysine isopeptide (Lys-Gly)

Acetylation; ADP-ribosylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disulfide bond; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005813; C:centrosome; ISS:UniProtKB.
GO:0005737; C:cytoplasm; ISS:UniProtKB.
GO:0005730; C:nucleolus; IDA:RGD.
GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IDA:RGD.
GO:0019899; F:enzyme binding; IDA:RGD.
GO:0051059; F:NF-kappaB binding; ISS:UniProtKB.
GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; IPI:RGD.
GO:0004860; F:protein kinase inhibitor activity; ISS:UniProtKB.
GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO:0051082; F:unfolded protein binding; ISS:UniProtKB.
GO:0007569; P:cell aging; ISS:UniProtKB.
GO:0007098; P:centrosome cycle; ISS:UniProtKB.
GO:0006281; P:DNA repair; ISS:UniProtKB.
GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
GO:0044387; P:negative regulation of protein kinase activity by regulation of protein phosphorylation; ISS:UniProtKB.
GO:0043085; P:positive regulation of catalytic activity; IDA:RGD.
GO:0045740; P:positive regulation of DNA replication; IDA:RGD.
GO:0045727; P:positive regulation of translation; ISS:UniProtKB.
GO:0051260; P:protein homooligomerization; IDA:RGD.
GO:0046599; P:regulation of centriole replication; ISS:UniProtKB.
GO:0060735; P:regulation of eIF2 alpha phosphorylation by dsRNA; ISS:UniProtKB.
GO:0032071; P:regulation of endodeoxyribonuclease activity; ISS:UniProtKB.
GO:0060699; P:regulation of endoribonuclease activity; ISS:UniProtKB.
GO:0043523; P:regulation of neuron apoptotic process; IMP:RGD.

IPR004301; Nucleoplasmin.
IPR024057; Nucleoplasmin_core_dom.