UniProt Accession

 SYYC_YEAST; P36421; D6VUW8 

Genbank Protein ID

 L12221; X71998; X99074; Z72970; U13015; BK006941 

Genbank Nucleotide ID

 AAB59329.1; CAA67529.1; CAA97211.1; AAA61641.1; DAA08279.1 

Protein Name

 Tyrosine--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

 Tyrosyl-tRNA synthetase; TyrRS 

Gene Name

 TYS1 

Gene Synonyms/Alias

 MGM104; YGR185C; G7522 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
99	EVVNYRAKYYELTIK	acetylation	[1]
118	SINVPIEKLKFVVGS	acetylation	[1]
150	IVSQNDAKRAGADVV	ubiquitination	[2]
194	FGGVDQRKIFVLAEE	ubiquitination	[2]
236	SASDPNSKIDLLEEP	acetylation	[1]
244	IDLLEEPKQVKKKIN	acetylation	[1]
295	FFIDRPEKFGGPITY	ubiquitination	[2]
303	FGGPITYKSFEEMKL	acetylation	[1]
313	EEMKLAFKEEKLSPP	ubiquitination	[2]
353	EFQEASEKGYPVATP	acetylation	[1]
353	EFQEASEKGYPVATP	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). The specificity determinants on tRNA(Tyr) are the base pair C1-G72, the discriminator residue A73, and the three anticodon bases G34, U35 and A36. Also involved in nuclear tRNA export. 

Sequence Annotation

 MOTIF 48 56 "HIGH" region.
 MOTIF 227 231 "KMSKS" region.
 MOTIF 360 378 Nuclear localization signal.
 BINDING 43 43 Tyrosine (By similarity).
 BINDING 170 170 Tyrosine (By similarity).
 BINDING 174 174 Tyrosine (By similarity).
 BINDING 177 177 Tyrosine (By similarity).
 BINDING 192 192 Tyrosine (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 235 235 Phosphoserine.
 MOD_RES 359 359 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 394 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IMP:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004831; F:tyrosine-tRNA ligase activity; IDA:SGD.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IDA:SGD. 

Interpro

 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002307; Tyr-tRNA-ligase.
 IPR023617; Tyr-tRNA-ligase_arc/euk-type. 

Pfam

 PF00579; tRNA-synt_1b 

SMART

  

PROSITE

 PS00178; AA_TRNA_LIGASE_I 

PRINTS

 PR01040; TRNASYNTHTYR.