CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000535
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable NAD-dependent malic enzyme 4 
Protein Synonyms/Alias
 NAD-ME 4 
Gene Name
 ytsJ 
Gene Synonyms/Alias
 BSU29220 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
45PGVAEPCKDIHEDINacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
  
Sequence Annotation
 NP_BIND 73 80 NAD (By similarity).
 ACT_SITE 36 36 Proton donor (By similarity).
 ACT_SITE 91 91 Proton acceptor (By similarity).
 METAL 133 133 Divalent metal cation (By similarity).
 METAL 134 134 Divalent metal cation (By similarity).
 BINDING 159 159 NAD (By similarity).
 BINDING 286 286 NAD (By similarity).  
Keyword
 Complete proteome; Metal-binding; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 410 AA 
Protein Sequence
MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED INKVYDYTMK 60
GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG VDAFPIALNT NDVDKIVETV 120
KLLEPTFGGV NLEDIAAPNC FIIEERLKKE TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK 180
SMSSIKVVAN GAGAAGIAII KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN 240
QDRKDGSLKD VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG 300
ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS LVSEDELSAD 360
YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE KTRKLTIIGE 410 
Gene Ontology
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:InterPro. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.