CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023585
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase 
Protein Synonyms/Alias
 UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine 2-epimerase (hydrolyzing); UDP-GlcNAc-2-epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase; N-acetylmannosamine kinase; ManAc kinase 
Gene Name
 GNE 
Gene Synonyms/Alias
 GLCNE 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24CNRADYSKLAPIMFGubiquitination[1, 2]
210MWLGDDVKSKDYIVAubiquitination[3]
385DLQEPLQKKFCFPPVubiquitination[3]
440KYTQFNPKTYEERINubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Regulates and initiates biosynthesis of N- acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. 
Sequence Annotation
 NP_BIND 411 420 ATP.
 NP_BIND 543 552 ATP.
 REGION 1 ? UDP-N-acetylglucosamine 2-epimerase.
 REGION 406 722 N-acetylmannosamine kinase.
 ACT_SITE 517 517
 METAL 569 569 Zinc.
 METAL 579 579 Zinc.
 METAL 581 581 Zinc.
 METAL 586 586 Zinc.
 BINDING 477 477 Substrate.
 BINDING 489 489 Substrate.
 BINDING 517 517 Substrate.
 BINDING 566 566 Substrate.
 BINDING 569 569 Substrate.
 BINDING 588 588 Substrate.  
Keyword
 3D-structure; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Disease mutation; Hydrolase; Kinase; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
MEKNGNNRKL RVCVATCNRA DYSKLAPIMF GIKTEPEFFE LDVVVLGSHL IDDYGNTYRM 60
IEQDDFDINT RLHTIVRGED EAAMVESVGL ALVKLPDVLN RLKPDIMIVH GDRFDALALA 120
TSAALMNIRI LHIEGGEVSG TIDDSIRHAI TKLAHYHVCC TRSAEQHLIS MCEDHDRILL 180
AGCPSYDKLL SAKNKDYMSI IRMWLGDDVK SKDYIVALQH PVTTDIKHSI KMFELTLDAL 240
ISFNKRTLVL FPNIDAGSKE MVRVMRKKGI EHHPNFRAVK HVPFDQFIQL VAHAGCMIGN 300
SSCGVREVGA FGTPVINLGT RQIGRETGEN VLHVRDADTQ DKILQALHLQ FGKQYPCSKI 360
YGDGNAVPRI LKFLKSIDLQ EPLQKKFCFP PVKENISQDI DHILETLSAL AVDLGGTNLR 420
VAIVSMKGEI VKKYTQFNPK TYEERINLIL QMCVEAAAEA VKLNCRILGV GISTGGRVNP 480
REGIVLHSTK LIQEWNSVDL RTPLSDTLHL PVWVDNDGNC AALAERKFGQ GKGLENFVTL 540
ITGTGIGGGI IHQHELIHGS SFCAAELGHL VVSLDGPDCS CGSHGCIEAY ASGMALQREA 600
KKLHDEDLLL VEGMSVPKDE AVGALHLIQA AKLGNAKAQS ILRTAGTALG LGVVNILHTM 660
NPSLVILSGV LASHYIHIVK DVIRQQALSS VQDVDVVVSD LVDPALLGAA SMVLDYTTRR 720
IY 722 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009384; F:N-acylmannosamine kinase activity; TAS:ProtInc.
 GO:0008761; F:UDP-N-acetylglucosamine 2-epimerase activity; TAS:ProtInc.
 GO:0007155; P:cell adhesion; TAS:ProtInc.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
 GO:0006045; P:N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0046380; P:N-acetylneuraminate biosynthetic process; IEA:InterPro.
 GO:0006054; P:N-acetylneuraminate metabolic process; TAS:ProtInc.
 GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IEA:InterPro. 
Interpro
 IPR001312; Hexokinase.
 IPR000600; ROK.
 IPR020004; UDP-GlcNAc_Epase.
 IPR003331; UDP_GlcNAc_Epimerase_2. 
Pfam
 PF02350; Epimerase_2
 PF00480; ROK 
SMART
  
PROSITE
 PS01125; ROK 
PRINTS
 PR00475; HEXOKINASE.