UniProt Accession

 H2AY_MOUSE; Q9QZQ8; Q91VZ2; Q9QZQ7 

Genbank Protein ID

 AF171080; AF171081; AB071988; BC006955 

Genbank Nucleotide ID

 AAD53745.1; AAD53746.1; BAB68541.1; AAH06955.1 

Protein Name

 Core histone macro-H2A.1 

Protein Synonyms/Alias

 Histone macroH2A1; mH2A1; H2A.y; H2A/y 

Gene Name

 H2afy 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
116	IHPELLAKKRGSKGK	acetylation	[1]
116	IHPELLAKKRGSKGK	ubiquitination	[2]
123	KKRGSKGKLEAIITP	acetylation	[1, 3]
123	KKRGSKGKLEAIITP	ubiquitination	[2]
189	GFTVLSTKSLFLGQK	ubiquitination	[2]
285	SPVWGADKCEELLEK	acetylation	[1, 3]
292	KCEELLEKTVKNCLA	acetylation	[3]
292	KCEELLEKTVKNCLA	ubiquitination	[2]
307	LADDRKLKSIAFPSI	ubiquitination	[2]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. 

Sequence Annotation

 DOMAIN 2 117 Histone H2A.
 DOMAIN 184 370 Macro.
 MOD_RES 18 18 N6-methyllysine (By similarity).
 MOD_RES 129 129 Phosphothreonine.
 MOD_RES 170 170 Phosphoserine (By similarity).
 MOD_RES 173 173 Phosphoserine (By similarity).
 MOD_RES 178 178 Phosphothreonine (By similarity).
 CROSSLNK 116 116 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 117 117 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Alternative splicing; Chromatin regulator; Chromosome; Complete proteome; DNA-binding; Isopeptide bond; Methylation; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 372 AA 

Protein Sequence

Gene Ontology

 GO:0001740; C:Barr body; ISO:MGI.
 GO:0005813; C:centrosome; TAS:MGI.
 GO:0000793; C:condensed chromosome; IDA:MGI.
 GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0007549; P:dosage compensation; ISO:MGI.
 GO:0006334; P:nucleosome assembly; IEA:InterPro. 

Interpro

 IPR002589; A1pp.
 IPR021171; Core_histone_macro-H2A.
 IPR009072; Histone-fold.
 IPR007125; Histone_core_D.
 IPR002119; Histone_H2A. 

Pfam

 PF00125; Histone
 PF01661; Macro 

SMART

 SM00506; A1pp
 SM00414; H2A 

PROSITE

 PS51154; MACRO 

PRINTS

 PR00620; HISTONEH2A.