CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006667
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lon protease homolog, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 PIM1 
Gene Synonyms/Alias
 LON; YBL022C; YBL0440 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
471NIEHRLEKSLLVLKKacetylation[1]
525GIDDGRDKLIDTYKEacetylation[1]
538KERIKSLKLPDSVQKacetylation[1]
584IPWGKHSKEQYSIPRacetylation[1]
883TKDNNSEKTSDDIEAubiquitination[2]
904INVSISQKNLKDYVGacetylation[1]
907SISQKNLKDYVGPPVacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner. Endogenous substrates include ACO2, ILV1, ILV2, LSC1, LYS4 and several oxidized proteins. 
Sequence Annotation
 DOMAIN 183 478 Lon.
 NP_BIND 632 639 ATP (By similarity).
 ACT_SITE 1015 1015 By similarity.
 ACT_SITE 1058 1058 By similarity.  
Keyword
 ATP-binding; Complete proteome; Direct protein sequencing; DNA-binding; Hydrolase; Mitochondrion; Nucleotide-binding; Protease; Reference proteome; Serine protease; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1133 AA 
Protein Sequence
MLRTRTTKTL STVARTTRAI QYYRSIAKTA AVSQRRFAST LTVRDVENIK PSHIIKSPTW 60
QEFQHQLKDP RYMEHFAQLD AQFARHFMAT NSGKSILAKD DSTSQKKDED VKIVPDEKDT 120
DNDVEPTRDD EIVNKDQEGE ASKNSRSSAS GGGQSSSSRS DSGDGSSKQK PPKDVPEVYP 180
QMLALPIARR PLFPGFYKAV VISDERVMKA IKEMLDRQQP YIGAFMLKNS EEDTDVITDK 240
NDVYDVGVLA QITSAFPSKD EKTGTETMTA LLYPHRRIKI DELFPPNEEK EKSKEQAKDT 300
DTETTVVEDA NNPEDQESTS PATPKLEDIV VERIPDSELQ HHKRVEATEE ESEELDDIQE 360
GEDINPTEFL KNYNVSLVNV LNLEDEPFDR KSPVINALTS EILKVFKEIS QLNTMFREQI 420
ATFSASIQSA TTNIFEEPAR LADFAAAVSA GEEDELQDIL SSLNIEHRLE KSLLVLKKEL 480
MNAELQNKIS KDVETKIQKR QREYYLMEQL KGIKRELGID DGRDKLIDTY KERIKSLKLP 540
DSVQKIFDDE ITKLSTLETS MSEFGVIRNY LDWLTSIPWG KHSKEQYSIP RAKKILDEDH 600
YGMVDVKDRI LEFIAVGKLL GKVDGKIICF VGPPGVGKTS IGKSIARALN RKFFRFSVGG 660
MTDVAEIKGH RRTYIGALPG RVVQALKKCQ TQNPLILIDE IDKIGHGGIH GDPSAALLEV 720
LDPEQNNSFL DNYLDIPIDL SKVLFVCTAN SLETIPRPLL DRMEVIELTG YVAEDKVKIA 780
EQYLVPSAKK SAGLENSHVD MTEDAITALM KYYCRESGVR NLKKHIEKIY RKAALQVVKK 840
LSIEDSPTSS ADSKPKESVS SEEKAENNAK SSSEKTKDNN SEKTSDDIEA LKTSEKINVS 900
ISQKNLKDYV GPPVYTTDRL YETTPPGVVM GLAWTNMGGC SLYVESVLEQ PLHNCKHPTF 960
ERTGQLGDVM KESSRLAYSF AKMYLAQKFP ENRFFEKASI HLHCPEGATP KDGPSAGVTM 1020
ATSFLSLALN KSIDPTVAMT GELTLTGKVL RIGGLREKAV AAKRSGAKTI IFPKDNLNDW 1080
EELPDNVKEG LEPLAADWYN DIFQKLFKDV NTKEGNSVWK AEFEILDAKK EKD 1133 
Gene Ontology
 GO:0042645; C:mitochondrial nucleoid; IBA:RefGenome.
 GO:0005524; F:ATP binding; IBA:RefGenome.
 GO:0004176; F:ATP-dependent peptidase activity; IMP:SGD.
 GO:0070361; F:mitochondrial light strand promoter anti-sense binding; IBA:RefGenome.
 GO:0043565; F:sequence-specific DNA binding; IEA:HAMAP.
 GO:0004252; F:serine-type endopeptidase activity; IEA:HAMAP.
 GO:0003697; F:single-stranded DNA binding; IBA:RefGenome.
 GO:0003727; F:single-stranded RNA binding; IBA:RefGenome.
 GO:0034599; P:cellular response to oxidative stress; IBA:RefGenome.
 GO:0051131; P:chaperone-mediated protein complex assembly; IGI:SGD.
 GO:0006515; P:misfolded or incompletely synthesized protein catabolic process; IMP:SGD.
 GO:0007005; P:mitochondrion organization; IBA:RefGenome.
 GO:0070407; P:oxidation-dependent protein catabolic process; IBA:RefGenome.
 GO:0051260; P:protein homooligomerization; IBA:RefGenome.
 GO:0090296; P:regulation of mitochondrial DNA replication; IEA:HAMAP.
 GO:0001666; P:response to hypoxia; IBA:RefGenome. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR004815; Lon_bac/euk-typ.
 IPR027065; Lon_Prtase.
 IPR027503; lonm_euk.
 IPR027417; P-loop_NTPase.
 IPR008269; Pept_S16_C.
 IPR003111; Pept_S16_N.
 IPR008268; Peptidase_S16_AS.
 IPR015947; PUA-like_domain.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr. 
Pfam
 PF00004; AAA
 PF02190; LON
 PF05362; Lon_C 
SMART
 SM00382; AAA
 SM00464; LON 
PROSITE
 PS01046; LON_SER 
PRINTS