UniProt Accession

 RPB7_YEAST; P34087; D6VT36 

Genbank Protein ID

 S59775; U32274; BK006938 

Genbank Nucleotide ID

 AAC60558.1; AAB64844.1; DAA12246.1 

Protein Name

 DNA-directed RNA polymerase II subunit RPB7 

Protein Synonyms/Alias

 RNA polymerase II subunit B7; B16 

Gene Name

 RPB7 

Gene Synonyms/Alias

 YDR404C; D9509.22 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
29	MKQYLKTKLLEEVEG	ubiquitination	[1]
80	KYRAVVFKPFKGEVV	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; DNA-directed RNA polymerase; Nucleus; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 171 AA 

Protein Sequence

Gene Ontology

 GO:0000932; C:cytoplasmic mRNA processing body; IDA:SGD.
 GO:0005665; C:DNA-directed RNA polymerase II, core complex; IDA:SGD.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0003727; F:single-stranded RNA binding; IDA:SGD.
 GO:0000291; P:nuclear-transcribed mRNA catabolic process, exonucleolytic; IGI:SGD.
 GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; IMP:SGD.
 GO:0045948; P:positive regulation of translational initiation; IMP:SGD.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:SGD. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR003029; Rbsml_prot_S1_RNA-bd_dom.
 IPR022967; RNA-binding_domain_S1.
 IPR005576; RNA_pol_Rpb7_N. 

Pfam

 PF00575; S1
 PF03876; SHS2_Rpb7-N 

SMART

 SM00316; S1 

PROSITE

  

PRINTS